FB2024_03 , released June 25, 2024
Allele: Dmel\muskelinGLC01768
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General Information
Symbol
Dmel\muskelinGLC01768
Species
D. melanogaster
Name
FlyBase ID
FBal0292022
Feature type
allele
Associated gene
Dmel\muskelin
Associated Insertion(s)
Carried in Construct
P{TRiP.GLC01768}
Also Known As
muskelin RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Ni et al., 2010.12.1)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ni et al., 2010.12.1)
Mutagen
in vitro construct
(Ni et al., 2010.12.1)
Progenitor genotype
Carried in construct
P{TRiP.GLC01768}
Cytology
Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-GLC01768
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Down-regulation of muskelin, through expression of muskelinGLC01768 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not visibly affect earlier stages of oogenesis, but results in persistence of nurse cell nuclei in stage 14 egg chambers. Approximately 65% of stage 14 egg chambers in the control lack nurse cell nuclei altogether or contain only one small, condensed nurse cell nucleus.

      Approximately 79% of stage 14 egg chambers depleted of muskelin contain at least one large nurse cell nucleus compared with ~35% in the control. Despite the presence of nurse cell nuclei in stage 14 egg chambers depleted of muskelin, the oocyte assumes the secondary metaphase I arrest. The metaphase I plate displays a normal configuration in 98.6% of control stage 14 oocytes compared with 96.8% in Scer\GAL4mat.αTub67C.T:Hsim\VP16->muskelinGLC01768 flies.

      Eggs resulting from Scer\GAL4mat.αTub67C.T:Hsim\VP16->muskelinGLC01768 treatment are fertile, producing viable embryos.

      (Kronja et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      muskelinGLC01768
      Name Synonyms
      Secondary FlyBase IDs
        References (3)