UASt regulates expression of wild type Hsap\GBA tagged with Tag:MYC and Tag:polyHis.
The expression of Hsap\GBAUAS.Tag:MYC,Tag:polyHis under the control of Scer\GAL4Ddc.PL does not significantly affect the adult lifespan, climbing ability, or number of dopaminergic neurons (total neurons and PPL1, PPM1/2 and PPM3 cluster neurons), as compared to controls.
Scer\GAL4da.G32-mediated expression of Hsap\GBAScer\UAS.T:Hsap\MYC,T:Zzzz\His6 results in decreased survival from L3 larva to pupa and again from pupa to adult, compared to controls.
Scer\GAL4Ddc.PL-mediated expression of Hsap\GBAScer\UAS.T:Hsap\MYC,T:Zzzz\His6 does not result in significant locomotion dysfunction (measured in a climbing assay) at 22 days of age, compared to controls.