FB2024_03 , released June 25, 2024
Allele: Ecol\lexAp65.iav
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General Information
Symbol
Ecol\lexAp65.iav
Species
E. coli
Name
FlyBase ID
FBal0291606
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
PBac{iav-lexA::p65}
Also Known As
iav-LexA
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Shearin et al., 2013)
Progenitor genotype
Carried in construct
PBac{iav-lexA::p65}
Cytology
Description

iav regulatory sequences control expression of a lexA::p65 driver. 3'UTR sequences from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene are present downstream of the coding sequence.

(Shearin et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
iav
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      chordotonal neuron
      (Shearin et al., 2013)
      vch1 neuron
      (Shearin et al., 2013)
      vch2 neuron
      (Shearin et al., 2013)
      lch1 neuron
      (Shearin et al., 2013)
      larval abdominal lch5 neuron
      (Shearin et al., 2013)
      adult stage
      mechanosensory neuron of femoral chordotonal organ
      (Tuthill and Wilson, 2016)
      adult ventral nerve cord | restricted
      (Tuthill and Wilson, 2016)
      Additional Information
      Statement
      Reference

      EcollexAp65.iav drives expression exclusively in chordotonal organs..

      (Shearin et al., 2013)
       
      Marker for
      mechanosensory neuron of femoral chordotonal organ
      (Tuthill and Wilson, 2016)
      Reflects expression of
      iav
      (Shearin et al., 2013)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Acute optogenetic activation (using Zzzz\ChRChrimson.IVS.Syn21.lexAop.tdTomato with 530 nm (green) light activation) of Ecol\lexAp65.iav neurons in the ipsilateral prothoracic femur hyperpolarizes Scer\GAL4GMR13D11 (adult intersegmental mechanosensory system) neurons and can suppress the excitation of Scer\GAL4GMR13D11 neurons caused by mechanical leg bristle stimulation, compared to controls. There is no effect of this optogenetic activation on Scer\GAL4GMR69C05 (adult midline local mechanosensory system) or Scer\GAL4GMR18G08 (adult midline bilateral mechanosensory system) neurons.

      (Tuthill and Wilson, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lexAiav.T:Hsap\RELA
      Ecol\lexAp65.iav
      Name Synonyms
      Secondary FlyBase IDs
        References (6)