Amino acid replacement: S119A.
UASt regulatory sequences drive expression of mutated Rok coding sequences where 5 serine residues within the kinase domain and near/within the Rho-binding domain have been mutated to alanines.
Amino acid replacement: S129A.
Amino acid replacement: S307A.
Amino acid replacement: S753A.
Amino acid replacement: S908A.
lethal - all die before end of pupal stage, with Scer\GAL4GMR.PU
Expression of rok5SA.Scer\UAS under the control of Scer\GAL4GMR.PU results in pupal lethality.