A 3271 base pair fragment from the flanking non-coding or intronic region of sc is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus with 685 nm (red) light) of Ecol\lexAGMR15A01 cells in adult males significantly increases wing extensions towards other males during, but not after, illumination and significantly increases aggressive lunging behavior towards other males after illumination, compared to controls.
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAGMR15A01 has abnormal courtship behavior | conditional phenotype, enhanceable by Hsap\KCNJ2UAS.EGFP/Scer\GAL4Tk
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAGMR15A01 has abnormal behavior | adult stage | conditional phenotype, suppressible | partially by Hsap\KCNJ2UAS.EGFP/Scer\GAL4Tk
Inhibition of Scer\GAL4Tk cells using Hsap\KCNJ2UAS.EGFP significantly increases wing extensions towards other males after, but not during, light activation of Ecol\lexAGMR15A01 cells using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, compared to activation alone.
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.