FlyBase Allele Report: Dmel\zfh2[EAB]

General Information

Symbol

Dmel\zfh2^EAB

Species

D. melanogaster

Name

FlyBase ID

FBal0288420

Feature type

allele

Associated gene

Dmel\zfh2

Associated Insertion(s)

Mi{Hto-WP}zfh2^EAB

Carried in Construct

Also Known As

UAS-zfh2

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

minos activity

Nature of the Allele

Allele class

Mutagen

minos activity

(Perea et al., 2013)

Progenitor genotype

Associated Insertion(s)

Mi{Hto-WP}zfh2^EAB

Cytology

Description

Insertion of a Mi{Hto-WP} element upstream of zfh2, at coordinate 4:515046 (release 5 genome). The HTO exon in the Mi{Hto-WP} element splices to the zfh2 exon that begins at coordinate 5:536208 . This zfh2 exon consists of 5'UTR, but is in frame with the zfh2 codon in the next exon. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP, linker sequence translated from the zfh2 5'UTR exon (KTPSPVRGNYAIDSINWSNNGNDDGSP) and the zfh2 coding sequence.

(Perea et al., 2013)

Allele components

Component

Use(s)

Inserted element

Mi{Hto-WP}

protein trap

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Tagged with

mCherry

red fluorescent protein

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

4:494,420..494,420 [+]

(Pranoto et al., 2023, Meng et al., 2020, Rojas Villa et al., 2019, Javeed et al., 2015, Guarner et al., 2014, Perea et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates intestinal cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

decreased occurrence of cell division | third instar larval stage, with Scer\GAL4^hh.PU

(Perea et al., 2013)

increased cell growth rate | adult stage, with Scer\GAL4^Su(H).GBE

(Meng et al., 2020)

increased cell size | adult stage, with Scer\GAL4^esg.PU

(Rojas Villa et al., 2019)

increased cell size | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

increased occurrence of cell division | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^hh.PU

(Perea et al., 2013)

viable

(Perea et al., 2013)

visible, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

visible, with Scer\GAL4^GMR.PU

(Javeed et al., 2015, Perea et al., 2013)

visible, with Scer\GAL4^nub-AC-62

(Perea et al., 2013)

visible, with Scer\GAL4^salm.EPv

(Perea et al., 2013)

Phenotype Manifest In

adult anterior midgut epithelium, with Scer\GAL4^esg.PU

(Rojas Villa et al., 2019)

adult gut, with Scer\GAL4^esg.PU

(Rojas Villa et al., 2019)

adult gut, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

adult posterior midgut epithelium, with Scer\GAL4^esg.PU

(Rojas Villa et al., 2019)

adult posterior midgut epithelium, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

chaeta | ectopic, with Scer\GAL4^salm.EPv

(Perea et al., 2013)

chorionic layer | somatic clone, with Scer\GAL4^Act5C.PP

(Javeed et al., 2015)

enteroblast, with Scer\GAL4^Su(H).GBE

(Meng et al., 2020)

enteroblast | adult stage, with Scer\GAL4^esg.PU

(Rojas Villa et al., 2019)

enteroblast | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

enteroblast | adult stage, with Scer\GAL4^T80

(Rojas Villa et al., 2019)

eye, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

eye, with Scer\GAL4^GMR.PU

(Javeed et al., 2015, Perea et al., 2013)

follicle cell | somatic clone, with Scer\GAL4^Act5C.PP

(Javeed et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

leg, with Scer\GAL4^ptc-559.1

(Javeed et al., 2015)

plasma membrane | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

wing, with Scer\GAL4^nub-AC-62

(Perea et al., 2013)

wing, with Scer\GAL4^salm.EPv

(Perea et al., 2013)

wing disc, with Scer\GAL4^hh.PU

(Perea et al., 2013)

wing hinge, with Scer\GAL4^nub-AC-62

(Perea et al., 2013)

Detailed Description

Statement

Reference

Expression of zfh2^EAB under the control of Scer\GAL4^Su(H).GBE (in combination with tub-Gal80[ts]) is sufficient to drive activation and growth of enteroblasts.

(Meng et al., 2020)

Expression of zfh2^EAB under the control of Scer\GAL4^esg.PU (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to an increase in the cell size of enteroblasts in adult guts when compared to controls.

Expression of zfh2^EAB under the control of Scer\GAL4^Su(H).GBE (in combination with tub-Gal80[ts]) leads to activation of enteroblasts (by decreasing cell circulatory and increasing number of cells with membrane protrusions); an increase in the cell size and cell volume of enteroblasts; and an increase in the proliferation of intestinal stem cells in adult guts when compared to controls.

Expression of zfh2^EAB under the control of Scer\GAL4^esg.PU (in combination with tub-Gal80[ts]) leads to formation of multilayered intestinal epithelium in the anterior and posterior midgut when compared to controls. Its expression under the control of Scer\GAL4^Su(H).GBE (in combination with tub-Gal80[ts]) also leads to formation of multilayered intestinal epithelium in posterior midgut.

Enteroblast clones expressing zfh2^EAB (under the control of Scer\GAL4^T80) do not exhibit terminal differentiation when compared to controls.

(Rojas Villa et al., 2019)

Expression of one copy of zfh2^EAB under the control of Scer\GAL4^GMR.PU produces flies that have mildly glazed (lacking clear separation of ommatidia) and rough eyes.

Expression of zfh2^EAB under the control of Scer\GAL4^Bx-MS1096 severely reduces growth of the wing blade.

Expression of zfh2^EAB under the control of Scer\GAL4^ptc-559.1 prevents the proper extension of the leg, and the tarsal region is not properly patterned or segmented.

Expression of zfh2^EAB under the control of Scer\GAL4^ey.PU results in a very reduced eye.

When zfh2^EAB-expressing clones are generated in follicle cells during mid-oogenesis under the control of Scer\GAL4^Act5C.PP) the nuclei are often displaced apically. Cell polarity is otherwise unaffected. There is increased secretion of eggshell at stage 10 compared with controls.

(Javeed et al., 2015)

Expression of zfh2^EAB under the control of Scer\GAL4^nub-AC-62 results in a reduced wing and a deleted wing hinge.

Flies expressing zfh2^EAB under the control of Scer\GAL4^salm.EPv have extra bristles in the wing blade.

BrdU incorporation is increased at both sides of the anterior/posterior compartment boundary and reduced in the posterior compartment in the wing discs of third instar larvae expressing zfh2^EAB under the control of Scer\GAL4^hh.PU.

(Perea et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Su(H).GBE, zfh2^EAB has abnormal cell shape | adult stage phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased cell size | adult stage phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased occurrence of cell division | adult stage phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased cell growth rate | adult stage phenotype, suppressible by Scer\GAL4^Su(H).GBE/Sox100B^GLV21021

(Meng et al., 2020)

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased cell size | adult stage phenotype, suppressible by Scer\GAL4^Su(H).GBE/gig^UAS.cUa/Tsc1^UAS.cUa

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased cell size | adult stage phenotype, suppressible by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased cell size | adult stage phenotype, non-suppressible by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has increased occurrence of cell division | adult stage phenotype, non-suppressible by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has abnormal cell shape | adult stage phenotype, non-suppressible by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Enhancer of

Statement

Reference

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of visible | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of abnormal cell number | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of hyperplasia | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Suppressor of

Statement

Reference

zfh2^EAB, Scer\GAL4^Su(H).GBE is a suppressor of decreased cell size | adult stage phenotype of Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

zfh2^EAB, Scer\GAL4^Su(H).GBE is a suppressor of decreased cell size | adult stage phenotype of InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^Su(H).GBE, zfh2^EAB has enteroblast | adult stage phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has adult gut phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has plasma membrane | adult stage phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has enteroblast | adult stage phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has adult gut phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has intestinal stem cell | adult stage phenotype, non-enhanceable by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^Su(H).GBE, zfh2^EAB has enteroblast phenotype, suppressible by Scer\GAL4^Su(H).GBE/Sox100B^GLV21021

(Meng et al., 2020)

Scer\GAL4^Su(H).GBE, zfh2^EAB has enteroblast | adult stage phenotype, suppressible by Scer\GAL4^Su(H).GBE/gig^UAS.cUa/Tsc1^UAS.cUa

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has adult gut phenotype, suppressible by Scer\GAL4^Su(H).GBE/gig^UAS.cUa/Tsc1^UAS.cUa

(Rojas Villa et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^Su(H).GBE, zfh2^EAB has enteroblast | adult stage phenotype, non-suppressible by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has adult gut phenotype, non-suppressible by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has enteroblast | adult stage phenotype, non-suppressible by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has adult gut phenotype, non-suppressible by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has intestinal stem cell | adult stage phenotype, non-suppressible by InR^K1409A.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Scer\GAL4^Su(H).GBE, zfh2^EAB has plasma membrane | adult stage phenotype, non-suppressible by Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Enhancer of

Statement

Reference

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of adult abdominal fat mass phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of ovary | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of enteroblast of posterior adult midgut epithelium | increased number phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

zfh2^EAB/Scer\GAL4^esg.PU is an enhancer of adult posterior midgut phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Suppressor of

Statement

Reference

zfh2^EAB, Scer\GAL4^Su(H).GBE is a suppressor of enteroblast | adult stage phenotype of Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

zfh2^EAB, Scer\GAL4^Su(H).GBE is a suppressor of adult gut phenotype of Ras85D^N17.UAS, Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

zfh2^EAB/Scer\GAL4^zfh2-LP30 partially rescues zfh2^RNAi.UAS

(Perea et al., 2013)

Comments

Expression of zfh2^EAB under the control of Scer\GAL4^zfh2-LP30 partially rescues the deletion of the wing hinge caused by expression of zfh2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^zfh2-LP30.

(Perea et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

56545

w^*; P{GAL4-ninaE.GMR}12/CyO; Mi{Hto-WP}zfh2^EAB

Notes on Origin

Discoverer

Comments

Generated using the hostile takeover (Hto) system.

(Javeed et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

zfh2^EAB

Name Synonyms

Secondary FlyBase IDs

References (6)

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