UAS regulatory sequences drive expression of an Tag:HA-tagged Dfd mutant. Conserved motifs 2 and 3 in the specificity module (SM) of the Dfd polypeptide are deleted. The region of the Dfd polypeptide from its YPWM motif through the N-terminal arm of its homeodomain, together with the intervening linker region, is referred to as the SM.
Expression of DfdΔ23.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS induces ectopic cirri in thoracic segments.