UAS regulates expression of a 't-peptide' comprising (from N to C terminus): a trypsin secretory signal sequence for targeting to the secretory pathway, a mature cleaved Pdf peptide sequence, a medium length hydrophilic linker sequence of 14 amino acids with an embedded Tag:MYC epitope tag. A GPI targeting sequence is lacking, and so the peptide is untethered.
Scer\GAL4tim.Scer\UAS-mediated expression of PdfScer\UAS.SEC.T:SS-trypsin.T:Hsap\MYC fails to induce free-running circadian locomotor rhythms in flies in constant darkness.