FlyBase Allele Report: Dmel\ERR[RNAi.UAS]

General Information

Symbol

Dmel\ERR^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0284080

Feature type

allele

Associated gene

Dmel\ERR

Associated Insertion(s)

Carried in Construct

P{UAS-ERR.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Lin et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lin et al., 2009)

Mutagen

in vitro construct

(Lin et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-ERR.RNAi}

Cytology

Description

UAS drives expression of a transcript encoding two copies of mir-6-1 modified to target ERR.

(Lin et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ERR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-fertile, with Scer\GAL4^c729

(Misra et al., 2017)

male semi-fertile, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

Phenotype Manifest In

axoneme, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

mitochondrial derivative, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

seminal vesicle, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

spermatozoon, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

sperm flagellum, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

testis, with Scer\GAL4^{CG13917-BG01278}

(Misra et al., 2017)

Detailed Description

Statement

Reference

Expression of ERR^{miRNA.Scer\UAS} under the control of Scer\GAL4^{CG13917-BG01278} or Scer\GAL4^c729 (but not Scer\GAL4^tj.PU, Scer\GAL4^c825, Scer\GAL4^sbb-BG01610, Scer\GAL4^bam.PU, Scer\GAL4^prd.RG1, Scer\GAL4^BG02820a, Scer\GAL4^nos.PU or Scer\GAL4^c855) significantly decreases male fertility (number of progeny produced by wild-type females mated to the knock-down males). Scer\GAL4^{CG13917-BG01278} driven expression of ERR^{miRNA.Scer\UAS} results in testicular deformity (underdeveloped testicular lobes and seminal vesicles) in adult males (although the frequency of the phenotype is rather low - around 20%). On the ultrastructural level, the males show several morphological defects: disorganization of the sperm bundles, decreased size of the mitochondrial derivatives as well as disruption of the axoneme structure in the sperm flagella.

(Misra et al., 2017)

Expression using Scer\GAL4^ey-OK107 does not produce a phenotype.

(Lin et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

44391

w^*; P{UAS-ERR.RNAi}attP16/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ERR^RNAi.UAS

ERR^{miRNA.Scer\UAS}

ERR^miRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close