The expression of Nrx-1ΔC.Scer\UAS under the control of Scer\GAL4elav.PU does not lead to significant changes in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.
Scer\GAL4RapGAP1-OK6/Nrx-1ΔC.UAS partially rescues Nrx-1273/Nrx-1Δ83
The expression of Nrx-1ΔC.Scer\UAS under the control of Scer\GAL4elav.PU rescues the decreased number of synaptic boutons observed at the Nrx-1273 homozygous third instar larval neuromuscular junction.
Expression of Nrx-1ΔC.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 fails to rescue the rapid synaptic depression, the reduced mean EJCs, and the reduced quantal content at the steady state seen in third instar Nrx-1273/Nrx-1Δ83 mutant NMJs under 1.8mM Ca[2+] conditions.
Expression of Nrx-1ΔPBD.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 restores the decreased initial EJC amplitude but not the the reduced mean EJCs at the steady state observed in third instar Nrx-1273/Nrx-1Δ83 mutant NMJs under 0.5mM Ca[2+] conditions.