FlyBase Allele Report: Dmel\ATP7[UAS.cBa]

General Information

Symbol

Dmel\ATP7^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Burke

FlyBase ID

FBal0281744

Feature type

allele

Associated gene

Dmel\ATP7

Associated Insertion(s)

Carried in Construct

P{UAS-ATP7.B}

Also Known As

UAS-ATP7-wt

Key Links

Transgenic product class

wild_type

(Burke et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Burke et al., 2008)

Mutagen

in vitro construct

(Burke et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-ATP7.B}

Cytology

Description

UASt regulatory sequences drive expression of ATP7.

(Burke et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ATP7

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of metal metabolism disorder

CEA

(Hartwig et al., 2021, Comstra et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of metal metabolism disorder

is ameliorated by Cog1^e02840

(Hartwig et al., 2021, Comstra et al., 2017)

is ameliorated by Cog8^HMC03886

(Hartwig et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Comstra et al., 2017)

male sterile (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

short lived (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

viable (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

visible (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

Phenotype Manifest In

eye (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

macrochaeta (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

microchaeta (with ATP7^ΔP17), with Scer\GAL4^ATP7.PB

(Burke et al., 2008)

neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Comstra et al., 2017)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Comstra et al., 2017)

Detailed Description

Statement

Reference

Expression of ATP7^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 reduces the cumulative synapse branch length and increases the bouton density in neuromuscular junctions in third instar larvae as compared to controls.

(Comstra et al., 2017)

Male flies expressing two copies of ATP7^Scer\UAS.cBa under the control of Scer\GAL4^ATP7.PB in a ATP7^ΔP17 mutant background are sterile, short lived and have a mild rough eye phenotype. Microchaetae and some microchaetae lack proper pigmentation.

(Burke et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ATP7^UAS.cBa, Scer\GAL4^elav-C155 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cog1[+]/Cog1^e02840

(Comstra et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

ATP7^UAS.cBa, Scer\GAL4^elav-C155 has neuromuscular junction | third instar larval stage phenotype, suppressible by Cog1[+]/Cog1^e02840

(Comstra et al., 2017)

ATP7^UAS.cBa, Scer\GAL4^elav-C155 has NMJ bouton | third instar larval stage phenotype, suppressible by Cog1[+]/Cog1^e02840

(Comstra et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased bouton density and reduced synaptic brach length of neuromuscular junctions in third instar larvae expressing ATP7^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 is suppressed by combination with a single copy of Cog1^e02840, the phenotype resembles that of Cog1^e02840 heterozygotes alone: the bouton density is comparable to wild-type but the cumulative branch length is increased.

(Comstra et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ATP7.PB/ATP7^UAS.cBa rescues ATP7^ΔP17

(Burke et al., 2008)

Comments

Expression of two copies of ATP7^Scer\UAS.cBa under the control of Scer\GAL4^ATP7.PB rescues the larval lethality associated with ATP7^ΔP17 mutant males. Rescue is significantly enhanced by raising the larvae on food supplemented with 1mM Cu. One copy of ATP7^Scer\UAS.cBa is insufficient for rescue.

(Burke et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ATP7^Scer\UAS.cBa

ATP7^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Burke

Secondary FlyBase IDs

References (3)

Report Sections

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