FlyBase Allele Report: Dmel\Atg4a[C98A.UAS]

General Information

Symbol

Dmel\Atg4a^C98A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0278391

Feature type

allele

Associated gene

Dmel\Atg4a

Associated Insertion(s)

Carried in Construct

P{UAS-Atg4a.C98A}

Also Known As

UAS-Atg4^C98A

Key Links

Genomic Maps

JBrowse

Transgenic product class

missense_variant

(Pircs et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Pircs et al., 2012)

Mutagen

in vitro construct

(Pircs et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Atg4a.C98A}

Cytology

Description

UASt regulatory sequences drive expression of a mutant Atg4a with a Cysteine 98 to Alanine amino acid substitution.

(Pircs et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg4a

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,081,685..1,081,686 [+]

Reported nucleotide change:

TG?GC

Reported amino acid change:

C98A

Comment:

Mutation carried on a transgenic construct. C98 (TGC) converted to A98 (GCC).

(Pircs et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | late third instar larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

Phenotype Manifest In

autophagosome | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

lysosome | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

Detailed Description

Statement

Reference

Expression of Atg4^{C98A.Scer\UAS} using Scer\GAL4^Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

Expression of Atg4^{C98A.Scer\UAS} using Scer\GAL4^Act5C.PP inhibits starvation-induced autophagic response in larval fat body cell somatic clones. Ultrastructural analysis reveals the lack of autolysosomes in fat body cells, although small autophagosome-like structures are occasionally observed.

(Pircs et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg4a^C98A.UAS, Scer\GAL4^Act5C.PP has abnormal starvation stress response | somatic clone | late third instar larval stage phenotype, suppressible by Atg8a^r4.mCherry, Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg4a^C98A.UAS, Scer\GAL4^Act5C.PP has embryonic/larval fat body | somatic clone | third instar larval stage phenotype, suppressible by Atg8a^r4.mCherry, Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Atg8a^{r4.T:Disc\RFP-mCherry} with Atg4^{C98A.Scer\UAS} under the control of Scer\GAL4^Act5C.PP restores both basal and starvation-induced autophagy.

(Pircs et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Atg4^{C98A.Scer\UAS}

Atg4a^{C98A.Scer\UAS}

Atg4a^C98A.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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