FlyBase Allele Report: Dmel\mir-1012[UAS.DsRed(Unk)]

General Information

Symbol

Dmel\mir-1012^{UAS.DsRed(Unk)}

Species

D. melanogaster

Name

FlyBase ID

FBal0277999

Feature type

allele

Associated gene

Dmel\mir-1012

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed-mir-1012.L}

Key Links

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

(Bejarano et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-DsRed-mir-1012.L}

Cytology

Description

UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-1012 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.

(Bejarano et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-1012

Tagged with

DsRed(Unk)

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^da.PU

(Mohammed et al., 2014)

lethal, with Scer\GAL4^ptc.PU

(Mohammed et al., 2014)

lethal, with Scer\GAL4^sd.PU

(Mohammed et al., 2014)

partially lethal - majority live, with Scer\GAL4^ey.PU

(Mohammed et al., 2014)

visible, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

visible, with Scer\GAL4^dpp.PU

(Bejarano et al., 2012)

visible, with Scer\GAL4^ptc-559.1

(Bejarano et al., 2012)

visible, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^dpp.PU

(Bejarano et al., 2012)

1st posterior cell, with Scer\GAL4^ptc-559.1

(Bejarano et al., 2012)

eye, with Scer\GAL4^GMR.PU

(Mohammed et al., 2014)

macrochaeta, with Scer\GAL4^Bx-MS1096

(Mohammed et al., 2014)

macrochaeta, with Scer\GAL4^sca.PU

(Mohammed et al., 2014)

mesothoracic tergum, with Scer\GAL4^Eq

(Mohammed et al., 2014)

microchaeta, with Scer\GAL4^Bx-MS1096

(Mohammed et al., 2014)

wing, with Scer\GAL4^Bx-MS1096

(Mohammed et al., 2014, Bejarano et al., 2012)

wing, with Scer\GAL4^sd.PU

(Mohammed et al., 2014)

wing cell, with Scer\GAL4^ptc.PU

(Mohammed et al., 2014)

wing margin, with Scer\GAL4^sd.PU

(Mohammed et al., 2014)

wing margin, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Detailed Description

Statement

Reference

Expression via Scer\GAL4^Bx-MS1096 results in missing notum macrochaetes and microchaetes in males, and curled, folded and blistered wings.

Expression via Scer\GAL4^GMR.PU results in rough eyes.

Expression via Scer\GAL4^elav.PU does not produce a phenotype.

Expression via Scer\GAL4^ptc.PU is lethal and results in space between L3 and L4 in the wing.

Expression via Scer\GAL4^Eq results in a misformed notum.

Expression via Scer\GAL4^sca.PU results in missing/extra macrochaetae.

Expression via Scer\GAL4^ey.PU results in rare lethality.

Expression via Scer\GAL4^sd.PU is lethal and results in almost no wing margin, and thinner/smaller wings.

(Mohammed et al., 2014)

Expression of mir-1012^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^Bx-MS1096 results in folded or smaller wings.

Expression of mir-1012^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^sd-SG29.1 results in lack of most of the wing margin.

Expression of mir-1012^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^ptc-559.1 results in a reduction in the 3-L4 intervein region.

(Bejarano et al., 2012)

Expression of mir-1012^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^dpp.PU results in undergrowth of the L3-L4 intervein region.

(Bejarano et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-1012^{Scer\UAS.T:Disc\RFP}

mir-1012^{UAS.DsRed(Unk)}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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