UASt regulatory sequences drive expression of a 500bp inverted repeat.
A significantly reduced number of males expressing Pka-C3NIG.6117R under the control of Scer\GAL4VP16.natalisin attempt copulation compared to controls.
Flies expressing Pka-C3NIG.6117R under the control of Scer\GAL4Appl.PU lack any overt defects and display normal brain development and integrity during adult life.
Expression of two copies (but not one copy) of Pka-C3NIG.6117R under the control of Scer\GAL4Appl.G1a results in flies exhibiting a significant decrease in performance in a phototaxis assay, as compared to controls.
Expression of Pka-C3NIG.6117R under the control of Scer\GAL4Appl.G1a (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a progressive increase in vacuolization in the brain, as compared to controls.
Pka-C3NIG.6117R is rescued by Scer\GAL4VP16.natalisin/Pka-C3UAS.cBa
Pka-C3NIG.6117R is not rescued by Pka-C3D397A.UAS.Tag:HA/Scer\GAL4VP16.natalisin
Co-expressing Pka-C3UAS.cBa (but not Pka-C3D397A.UAS.Tag:HA) rescues the reduced occurrence of copulation in males expressing Pka-C3NIG.6117R under the control of Scer\GAL4VP16.natalisin.