FlyBase Allele Report: Dmel\pum[UAS.cKa]

General Information

Symbol

Dmel\pum^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kim

FlyBase ID

FBal0268505

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

P{UAS-pum.K}

Key Links

Transgenic product class

cDNA

(Kim et al., 2012)

wild_type

(Kim et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kim et al., 2012)

wild_type

(Kim et al., 2012)

Mutagen

in vitro construct

(Kim et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-pum.K}

Cytology

Description

UAS regulatory sequences drive expression of a full length pum cDNA (LD44635).

(Kim et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

pum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^dpp.blk1

(Kim et al., 2012)

visible, with Scer\GAL4^sca-537.4

(Kim et al., 2012)

visible, with Scer\GAL4^Ser.PGF

(Kim et al., 2012)

Phenotype Manifest In

macrochaeta, with Scer\GAL4^sca-537.4

(Kim et al., 2012)

metathoracic tergum, with Scer\GAL4^sca-537.4

(Kim et al., 2012)

wing margin, with Scer\GAL4^Ser.PGF

(Kim et al., 2012)

wing vein, with Scer\GAL4^dpp.blk1

(Kim et al., 2012)

Detailed Description

Statement

Reference

Overexpression of pum^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1 results in wing vein loss.

Overexpression of pum^Scer\UAS.cKa under the control of Scer\GAL4^Ser.PGF results in distal wing notches.

Overexpression of pum^Scer\UAS.cKa in the notum under the control of Scer\GAL4^sca-537.4 eliminates macrochaetae.

(Kim et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Ser.PGF, pum^UAS.cKa has visible phenotype, non-enhanceable by Egfr^UAS.cUa, Scer\GAL4^Ser.PGF

(Kim et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, pum^UAS.cKa has visible phenotype, non-suppressible by Egfr^UAS.cUa, Scer\GAL4^Ser.PGF

(Kim et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^dpp.blk1/pum^UAS.cKa is a suppressor | partially of visible phenotype of Scer\GAL4^dpp.blk1, rl^Sem.MB.UAS

(Kim et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^dpp.blk1/pum^UAS.cKa is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4^dpp.blk1, rl^Sem.MB.UAS

(Kim et al., 2012)

pum^UAS.cKa, Scer\GAL4^Ser.PGF is a suppressor of wing vein | ectopic phenotype of Egfr^UAS.cUa, Scer\GAL4^Ser.PGF

(Kim et al., 2012)

pum^UAS.cKa, Scer\GAL4^sca-537.4 is a suppressor of metathoracic tergum phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

pum^UAS.cKa, Scer\GAL4^sca-537.4 is a suppressor of macrochaeta | ectopic phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-overexpression of pum^Scer\UAS.cKa with Egfr^Scer\UAS.cUa under the control of Scer\GAL4^Ser.PGF suppressed the development of ectopic wing veins resulting from the overexpression of Egfr^Scer\UAS.cUa alone generating wings that are indistinguishable from those overexpressing pum^Scer\UAS.cKa alone.

Co-overexpression of pum^Scer\UAS.cKa with rl^Sem.Scer\UAS under the control of Scer\GAL4^dpp.blk1 suppresses the ectopic wing veins caused by overexpression of rl^Sem.Scer\UAS alone.

Co-overexpression of pum^Scer\UAS.cKa and Egfr^Scer\UAS.cUa in the notum under the control of Scer\GAL4^sca-537.4 suppresses the Egfr^Scer\UAS.cUa-induced ectopic macrochaeta phenotype.

(Kim et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pum^Scer\UAS.cKa

pum^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kim

Secondary FlyBase IDs

References (1)

Report Sections

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