The effect on border cell cluster migration of expressing PvrScer\UAS.T:Avic\GFP under the control of Scer\GAL4c522 in a single border follicle cell in a EgfrdsRNA.slbo Pvf1EP1624 background (to remove endogenous guidance information) has been studied. These clusters are dynamic and display disordered movement including cluster tumbling. In those border cell clusters where the single PvrScer\UAS.T:Avic\GFP-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the PvrScer\UAS.T:Avic\GFP-expressing cell is in the back, the cluster on average moves relatively more backwards and when the PvrScer\UAS.T:Avic\GFP-expressing cell is on the side of the cluster, border cell migration is unaltered compared to control clusters without a PvrScer\UAS.T:Avic\GFP-expressing cell.
The effect on border cell cluster migration of expressing Pvr::Hsap\EGFRhE-P.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4c522 in a single border follicle cell in a EgfrdsRNA.slbo Pvf1EP1624 background (to remove endogenous guidance information) has been studied. Overall disordered, or tumbling border cell movements are seen in these clusters. In those border cell clusters where the single Pvr::Hsap\EGFRhE-P.Scer\UAS.T:Avic\GFP-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Pvr::Hsap\EGFRhE-P.Scer\UAS.T:Avic\GFP-expressing cell is in the back, the cluster on average moves relatively more backwards.