twi promoter sequences drive expression of RanGAP which is tagged at the N-terminal end with three copies of Tag:FLAG, Tag:BLRP and mCherry.
The P{twi-NTF} construct allows purification of nuclei from twi-expressing cells via in vivo biotin-labelling of the nuclear envelope followed by affinity purification of the nuclei.
The P{twi-NTF} construct allows purification of nuclei from twi-expressing cells via in vivo biotin-labelling of the nuclear envelope followed by affinity purification of the nuclei.