FlyBase Allele Report: Dmel\caz[UAS.x10.Tag:FLAG]

General Information

Symbol

Dmel\caz^{UAS.x10.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0265758

Feature type

allele

Associated gene

Dmel\caz

Associated Insertion(s)

Carried in Construct

P{10xUAS-caz.FLAG}

Also Known As

UAS-caz

Key Links

Transgenic product class

cDNA

(Wang et al., 2011)

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wang et al., 2011)

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

(Wang et al., 2011)

Progenitor genotype

Carried in construct

P{10xUAS-caz.FLAG}

Cytology

Description

Ten copies of UAS regulatory sequences drive expression of a caz cDNA fused to an N-terminal Tag:FLAG epitope tag.

(Wang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

caz

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 6

CEC

(Cacciottolo et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 6

modeled by caz²

(Frickenhaus et al., 2015)

modeled by caz^KO

(Frickenhaus et al., 2015)

ameliorates amyotrophic lateral sclerosis

modeled by caz³⁸³

(Baldwin et al., 2016)

model of amyotrophic lateral sclerosis type 6

is exacerbated by Gem3^ΔN.UAS

(Cacciottolo et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval motor neuron | third instar larval stage, with Scer\GAL4^CCAP.PU

(Baldwin et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

Detailed Description

Statement

Reference

Individuals expressing caz^{UAS.x10.Tag:FLAG} under the control of Scer\GAL4^elav-C155 do not show any obvious change in lifespan, as compared to controls. caz^KO hemizygous males expressing caz^{UAS.x10.Tag:FLAG} under the control of Scer\GAL4^elav-C155, however, exhibit significant decreases in lifespan and climbing ability (i.e. speed), as compared to controls.

(Mallik et al., 2018)

The expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^CCAP.PI leads to a decrease in axonal transport of NPY-positive vesicles (i.e. a significant decrease in anterograde transport and a significant increase in the stationary fraction) and of mitochondria (i.e. a significant decrease in retrograde transport and a significant increase in the stationary fraction), in third instar larval motor neurons, as compared to controls.

The expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^Toll-6-D42 does not lead to any obvious defects in viability or third instar larval locomotion, but induces a significant and progressive decrease in adult locomotion, as compared to controls.

(Baldwin et al., 2016)

Overexpression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} in motor neurons via Scer\GAL4^RapGAP1-OK6 induces neuromuscular junction (NMJ) expansion.

Overexpression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} has no effect on longevity.

(Wang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, caz^{UAS.x10.Tag:FLAG} has abnormal neuroanatomy phenotype, non-enhanceable by Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, caz^{UAS.x10.Tag:FLAG} has abnormal neuroanatomy phenotype, non-suppressible by Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PS/caz^{UAS.x10.Tag:FLAG} is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

Scer\GAL4^GMR.PS/caz^{UAS.x10.Tag:FLAG} is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

Scer\GAL4^GMR.PS/caz^{UAS.x10.Tag:FLAG} is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

caz^{UAS.x10.Tag:FLAG}/Scer\GAL4^Toll-6-D42 is a suppressor of partially lethal - majority die phenotype of TBPH¹/TBPH^Δ23

(Baldwin et al., 2016)

caz^{UAS.x10.Tag:FLAG}/Scer\GAL4^Toll-6-D42 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of TBPH¹/TBPH^Δ23

(Baldwin et al., 2016)

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} is a suppressor of partially lethal - majority die phenotype of Df(2R)BSC660/TBPH^Δ23

(Wang et al., 2011)

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} is a suppressor of short lived phenotype of Df(2R)BSC660/TBPH^Δ23

(Wang et al., 2011)

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} is a suppressor | partially of abnormal locomotor behavior phenotype of Df(2R)BSC660/TBPH^Δ23

(Wang et al., 2011)

NOT Suppressor of

Statement

Reference

caz^{UAS.x10.Tag:FLAG}/Scer\GAL4^Toll-6-D42 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of TBPH¹/TBPH^Δ23

(Baldwin et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, caz^{UAS.x10.Tag:FLAG} has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, caz^{UAS.x10.Tag:FLAG} has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Df(2R)BSC660/TBPH^Δ23, Scer\GAL4^RapGAP1-OK6

(Wang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PS/caz^{UAS.x10.Tag:FLAG} is a suppressor of eye phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

Scer\GAL4^GMR.PS/caz^{UAS.x10.Tag:FLAG} is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^CCAP.PU/caz^{UAS.x10.Tag:FLAG} is a non-suppressor of embryonic/larval motor neuron | third instar larval stage phenotype of TBPH¹/TBPH^Δ23

(Baldwin et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Neuronal overexpression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 in TBPH^Δ23/Df(2R)BSC660 mutants restores their eclosion rate and longevity to levels not significantly different from that of wild-type animals.

Although overexpression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 in TBPH^Δ23/Df(2R)BSC660 mutants significantly improves their locomotion velocity, these animals are still significantly slower than controls.

The level of NMJ expansion induced by the overexpression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} via Scer\GAL4^RapGAP1-OK6 is not affected by TBPH^Δ23/Df(2R)BSC660.

(Wang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^CCAP.PU/caz^{UAS.x10.Tag:FLAG} rescues caz³⁸³

(Baldwin et al., 2016)

caz^{UAS.x10.Tag:FLAG}/Scer\GAL4^Toll-6-D42 rescues caz³⁸³

(Baldwin et al., 2016)

Partially rescues

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} partially rescues caz²

(Mallik et al., 2018)

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} partially rescues caz^KO

(Mallik et al., 2018)

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} partially rescues caz²

(Frickenhaus et al., 2015)

Scer\GAL4^elav-C155/caz^{UAS.x10.Tag:FLAG} partially rescues caz^KO

(Frickenhaus et al., 2015)

Scer\GAL4^elav-C155, caz^{UAS.x10.Tag:FLAG}, CG32576^+t5.0 partially rescues Df(1)383

(Wang et al., 2011)

Comments

Expressing caz^{UAS.x10.Tag:FLAG} under the control of Scer\GAL4^elav-C155 rescues both caz² and caz[KO] hemizygous males' pupal lethality, but the resulting adults exhibit a significant decrease in climbing ability (i.e. speed) as compared to controls.

(Mallik et al., 2018)

The expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^CCAP.PI rescues the decreased axonal transport of mitochondria and (NPY-positive) vesicles observed in caz³⁸³ homozygous third instar larval motor neurons.

The expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^Toll-6-D42 rescues the semi-lethality, the decreased third instar larval crawling capacity and the decreased adult locomotor capacity observed in caz³⁸³ homozygotes.

(Baldwin et al., 2016)

Expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 partially rescues the lethality seen in caz² or caz^KO hemizygous males.

(Frickenhaus et al., 2015)

Expression of caz^{Scer\UAS.x10.T:Zzzz\FLAG} in the nervous system under the control of Scer\GAL4^elav-C155 rescues the adult viability of males hemizygous for the 'caz¹' mutant chromosome (Df(1)383 onto which a transgene containing CG32576^+t5.0 has been recombined to provide CG32576[+] function) to 83.7% of control levels. The locomotion defects seen in caz¹/Y adult escapers are also strongly, but not completely rescued in these animals, while longevity is completely rescued.

(Wang et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

caz^{Scer\UAS.x10.T:Zzzz\FLAG}

caz^{UAS.x10.Tag:FLAG}

caz^WT

(Baldwin et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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