FB2024_03 , released June 25, 2024
Allele: Dmel\FimCPTI100066
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General Information
Symbol
Dmel\FimCPTI100066
Species
D. melanogaster
Name
FlyBase ID
FBal0261376
Feature type
allele
Associated gene
Dmel\Fim
Associated Insertion(s)
PBac{544.SVS-1}FimCPTI100066
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
piggyBac activity
(FlyProt project., 2011.8.8)
Progenitor genotype
Associated Insertion(s)
PBac{544.SVS-1}FimCPTI100066
Cytology
Description

Protein trap insertion line.

(FlyProt project., 2011.8.8)
Allele components
Component
Use(s)
Inserted element
PBac{544.SVS-1}
Tagged with
Tag:StrepII
Venus
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Fim protein knock-down by using slmbαTub67C.vhhGFP4 in a FimCPTI100066 homozygous background results in assembly of a stable hexagonal network that does not change conformation over time, resulting in a significant delay of actomyosin ring formation and slower constriction kinetics than controls. On STED super-resolution microscopy, actin fiber fail to form a ring, remaining locked in a stable hexagonal array; the thickness of these actin fibers appears similar to controls.

      (Krueger et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Df(3R)Exel6218 homozygous embryos display increased/premature actomyosin constriction during cellularization as well as defective ventral furrow invagination during gastrulation, both of which are partially suppressed by the depletion of Fim protein by using slmbαTub67C.vhhGFP4 in a FimCPTI100066 homozygous background.

      (Krueger et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      FimCPTI100066
      Name Synonyms
      Secondary FlyBase IDs
        References (2)