UASt regulatory sequences drive expression of Hsap\TARDBP amino acid residues 80-414 (the N-terminal nuclear localization signal is deleted). The protein is tagged with Tag:MYC.
Eye degeneration used to model disease.
Expression of Hsap\TARDBPΔ1-79.Scer\UAS under the control of Scer\GAL4wor.PA induces the accumulation of ubiquitin conjugates in larval neuroblasts but does not cause neuroblast loss.
Expression of Hsap\TARDBPΔ1-79.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF results in a strong degenerative phenotype.