The overexpression of ana2Ubi.N.T:Avic\GFP does not cause centriole overduplication in spermatocytes or syncytial embryos.
Spermatocytes expressing ana2Ubi.N.T:Avic\GFP, in addition to the normal centriole pairs (doublets), exhibit centriole triplets, quadruplets, and even quintets.The extra centrioles in these clusters appear to be fully functional; they separated from one another by the end of meiosis I (as centriole doublets normally do), and the extra centrioles inherited by secondary spermatocytes result PCM and nucleated MT asters, and so form multipolar spindles during meiosis II. Centriole overduplication is not found in embryos or brain cells expressing ana2Ubi.N.T:Avic\GFP.
Unfertilized eggs laid by ana2Ubi.N.T:Avic\GFP females no not assemble centriole-like structures.
Co-expression of Sas-6Ubi.P.T:Avic\GFP and ana2Ubi.N.T:Avic\GFP does not perturb centriole length in wild-type wing disc cells.
Co-expression of Sas-6Ubi.P.T:Avic\GFP and ana2Ubi.N.T:Avic\GFP dramatically increases centriole length by approximately 60% in Cp110Δ wing disc cells.
Overexpression of ana2Ubi.N.T:Avic\GFP increases centriole length by approximately 60% in Cp110Δ wing disc cells.
Overexpression of ana2Ubi.N.T:Avic\GFP in a Cp110Δ mutant background drives centriole overduplication in spermatocytes. ana2 overexpression leads predominantly to the formation of rosette-like structures.
Overexpression of ana2Ubi.N.T:Avic\GFP in a Cp110Δ mutant syncytial embryos induces centriole overduplication.