FB2024_03 , released June 25, 2024
Allele: Dmel\magNIG.5932R
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General Information
Symbol
Dmel\magNIG.5932R
Species
D. melanogaster
Name
FlyBase ID
FBal0260741
Feature type
allele
Associated gene
Dmel\mag
Associated Insertion(s)
Carried in Construct
P{NIG.5932R}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
in vitro construct
(Japanese National Institute of Genetics, 2012.5.21)
Progenitor genotype
Carried in construct
P{NIG.5932R}
Cytology
Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-5932R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing magNIG.5932R under the control of Scer\GAL4Act5C.PI display elevated levels of cholesterol and decreased levels of triacylglycerol (TAG) compared to controls. Wild type levels of TAG, but not cholesterol, are restored by raising flies on a diet supplemented with pancreatin (a mixture of pancreatic enzymes).

      Flies expressing magNIG.5932R under the control of Scer\GAL4mex1.2.1 increased levels of both total cholesterol and cholesterol esters, whereas free cholesterol levels remain unchanged.

      Flies expressing magNIG.5932R under the control of Scer\GAL4bab1-Agal4-5 display decreased levels of triacylglycerol (TAG) compared to controls, whereas total cholesterol levels are unaffected.

      (Sieber and Thummel, 2012)

      Expression of CG5932NIG.5932R with Scer\GAL4Act5C.PI results in significant reduction of whole animal triacylglycerol levels.

      (Sieber and Thummel, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG5932NIG.5932R
      magNIG.5932R
      Name Synonyms
      Secondary FlyBase IDs
        References (3)