FlyBase Allele Report: Dmel\Opa1[RNAi.CDS.UAS]

General Information

Symbol

Dmel\Opa1^RNAi.CDS.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0258606

Feature type

allele

Associated gene

Dmel\Opa1

Associated Insertion(s)

Carried in Construct

P{UAS-Opa1.RNAi.CDS}

Key Links

Transgenic product class

RNAi_reagent

(Deng et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Deng et al., 2008)

Mutagen

in vitro construct

(Deng et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Opa1.RNAi.CDS}

Cytology

Description

UAS regulatory sequences drive expression of a miRNA targeted to the Opa1 coding region.

(Deng et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Opa1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate Charcot-Marie-Tooth disease type 2

modeled by Marf^R94Qlike.UAS

(El Fissi et al., 2018)

ameliorates Charcot-Marie-Tooth disease type 2

modeled by Marf^{R364Wlike.UAS}

(El Fissi et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased cell number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

short lived, with Scer\GAL4^tin.CΔ4

(Dorn et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Opa1^{miRNA.CDS.UAS} under the control of Scer\GAL4^pnt-14-94 results in decreased number of mature intermediate precursor cells and young neurons in the larval brain.

(Dubal et al., 2022)

Expression of Opa1^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^Feb36 results in an increase in the number of boutons at the neuromuscular junction compared to wild type.

Wandering third instar larvae expressing Opa1^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^Feb36 have a normal number of lipid droplets per ring gland.

(Sandoval et al., 2014)

Individuals expressing Opa1^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 exhibit a significant decrease in lifespan, as compared to controls.

Mitochondria tend to cluster in aggregates in cardiomyocytes of adults expressing opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4. Mitochondrial fragmentation is seen and mean mitochondrial size is decreased approximately 30% compared to wild type.

The heart tubes of adults expressing opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 are dilated compared to wild type and show impaired shortening with no change in the beating rate.

(Dorn et al., 2011)

Expression of opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^Mef2.PR results in mitochondrial fragmentation (they are smaller and rounder than normal) in the indirect flight muscles.

(Deng et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a non-enhancer of abnormal size | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Suppressor of

Statement

Reference

Opa1^RNAi.CDS.UAS/Scer\GAL4^Tub.PU is a suppressor | partially of increased size | somatic clone | oogenesis phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a suppressor of abnormal size | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a non-suppressor of abnormal size | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a non-enhancer of mitochondrion | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a non-enhancer of embryonic/larval motor neuron | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Suppressor of

Statement

Reference

Opa1^RNAi.CDS.UAS/Scer\GAL4^Tub.PU is a suppressor | partially of follicle cell | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a suppressor of mitochondrion | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a suppressor of embryonic/larval motor neuron | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a suppressor of mitochondrion phenotype of Drp1²/Drp1¹

(Rival et al., 2011)

Opa1^RNAi.CDS.UAS/Scer\GAL4^Mef2.PR is a suppressor of mitochondrion phenotype of Pink1⁵

(Deng et al., 2008)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a non-suppressor of mitochondrion | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS is a non-suppressor of embryonic/larval motor neuron | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^elav.PU is sufficient to restore a normal filamentous mitochondrial network in Drp1¹/Drp1² flies.

Expression of opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^elav.PU does not rescue the abnormal mitochondrial morphology of Df(3L)Pmi-PGRP-LD flies.

(Rival et al., 2011)

Expression of opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^Mef2.PR suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink1⁵ mutants.

(Deng et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67159

w^*; Bl¹/CyO; P{UAS-Opa1.RNAi.CDS}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Opa1^RNAi.CDS.UAS

Opa1^{miRNA.CDS.Scer\UAS}

Opa1^{miRNA.CDS.UAS}

opa1-like^{miRNA.CDS.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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