FlyBase Allele Report: Dmel\Ephrin[HMS01289]

General Information

Symbol

Dmel\Ephrin^HMS01289

Species

D. melanogaster

Name

FlyBase ID

FBal0257884

Feature type

allele

Associated gene

Dmel\Ephrin

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01289}

Also Known As

UAS-ephrin^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01289}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01289

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ChAT.7.4

(Liu et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR31C06

(Liu et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^Ln

(Liu et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ort.C1-4

(Liu et al., 2017)

abnormal neurophysiology | adult stage, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

abnormal sleep, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

decreased efficacy of memory | adult stage, with Scer\GAL4^ey-OK107

(Buhl et al., 2022)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^ChAT.7.4

(Liu et al., 2017)

axon | adult stage, with Scer\GAL4^GMR31C06

(Liu et al., 2017)

axon | adult stage, with Scer\GAL4^Ln

(Liu et al., 2017)

axon | adult stage, with Scer\GAL4^ort.C1-4

(Liu et al., 2017)

lamina monopolar neuron L4 | adult stage, with Scer\GAL4^ChAT.7.4

(Liu et al., 2017)

lamina monopolar neuron L4 | adult stage, with Scer\GAL4^GMR31C06

(Liu et al., 2017)

lamina monopolar neuron L4 | adult stage, with Scer\GAL4^Ln

(Liu et al., 2017)

lamina monopolar neuron L4 | adult stage, with Scer\GAL4^ort.C1-4

(Liu et al., 2017)

l-LNv neuron, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

medulla | adult stage, with Scer\GAL4^ChAT.7.4

(Liu et al., 2017)

medulla | adult stage, with Scer\GAL4^GMR31C06

(Liu et al., 2017)

medulla | adult stage, with Scer\GAL4^Ln

(Liu et al., 2017)

medulla | adult stage, with Scer\GAL4^ort.C1-4

(Liu et al., 2017)

Detailed Description

Statement

Reference

Expression of Ephrin^HMS01289 under the control of any of the following drivers: Scer\GAL4^GMR31C06, Scer\GAL4^Ln, Scer\GAL4^ort.C1-4 or Scer\GAL4^ChAT.7.4 (but not Scer\GAL4^repo, Scer\GAL4^Pan-R7 or Scer\GAL4^oc.1.6) results in the aberrant projection of axons of the L4 lamina neurons into adjacent columns in the adult medulla. Additionally, Scer\GAL4^Ln-driven expression of Ephrin^HMS01289 leads to extension of L4 axons into the M5 layer (instead of the M4 layer as in wild-type).

(Liu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1^Δ83

(Liu et al., 2017)

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has lamina monopolar neuron L4 | adult stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1^Δ83

(Liu et al., 2017)

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has axon | adult stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1^Δ83

(Liu et al., 2017)

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has medulla | adult stage phenotype, non-enhanceable by Nrx-1²⁷³/Nrx-1^Δ83

(Liu et al., 2017)

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has lamina monopolar neuron L4 | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has axon | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Ephrin^HMS01289, Scer\GAL4^GMR31C06 has medulla | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The impaired columnar restriction of L4 lamina neuron axons (which frequently invade adjacent medulla columns) observed in adult flies expressing Ephrin^HMS01289 under the control of Scer\GAL4^GMR31C06 is not worsened further by combination with either Nrx-1^Δ83/Nrx-1²⁷³ or Nlg4^KO10 in homozygous state - either of which on their own also causes defects in columnar restriction of the L4 axons.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34614