FlyBase Allele Report: Dmel\Pi3K59F[HMS00261]

General Information

Symbol

Dmel\Pi3K59F^HMS00261

Species

D. melanogaster

Name

FlyBase ID

FBal0257161

Feature type

allele

Associated gene

Dmel\Pi3K59F

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00261}

Also Known As

Vps34 RNAi, UAS-vps34RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00261}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00261

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

abnormal starvation stress response | larval stage, with Scer\GAL4^Cg.PU

(Xu et al., 2015)

decreased cell death | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

Phenotype Manifest In

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PU

(Xu et al., 2015)

embryonic/larval midgut | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

gastric caecum | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

Detailed Description

Statement

Reference

Third instar larvae expressing Pi3K59F^HMS00261 under the combined control of Scer\GAL4^sca.PU and Scer\GAL4^C57 do not show apparent defects in neurotransmission across the neuromuscular junctions (mEPSP and EPSP amplitudes, and quantal content), as compared to controls.

(Hauswirth et al., 2018)

Expression of Pi3K59F^HMS00261 under the control of Scer\GAL4^{Myo31DF-NP0001} results in defective midgut degradation after pupariation, with significantly increased size of the gastric caeca 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.

Expression of Pi3K59F^HMS00261 under the control of Scer\GAL4^cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

(Xu et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Pi3K59F^HMS00261/Scer\GAL4^αTub84B.PL is a non-suppressor of increased cell number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

Phenotype Manifest In