FlyBase Allele Report: Dmel\E(z)[HMS00066]

General Information

Symbol

Dmel\E(z)^HMS00066

Species

D. melanogaster

Name

FlyBase ID

FBal0257100

Feature type

allele

Associated gene

Dmel\E(z)

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00066}

Also Known As

E(z) RNAi, UAS-E(z)^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00066}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00066

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates adult T-cell leukemia/lymphoma

modeled by HTLV-1\Tax^UAS.Tag:MYC

(Akkouche et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst progenitor cell | increased number, with Scer\GAL4^A3.eya

(Eun et al., 2014)

cyst progenitor cell | increased number, with Scer\GAL4^C587

(Eun et al., 2014)

cyst progenitor cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

egg chamber, with Scer\GAL4^nanos.PG

(Iovino et al., 2013)

enteroendocrine cell | decreased number, with Scer\GAL4^esg.PU

(Tauc et al., 2021)

germarium region 2b, with Scer\GAL4^nanos.PG

(Iovino et al., 2013)

germinal proliferation center | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

karyosome, with Scer\GAL4^nanos.PG

(Iovino et al., 2013)

male germline cell, with Scer\GAL4^C587

(Eun et al., 2014)

male germline stem cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

oocyte nucleus, with Scer\GAL4^nanos.PG

(Iovino et al., 2013)

primary gonial cell | spermatogenesis, with Scer\GAL4^C587

(Zhang et al., 2017)

testis, with Scer\GAL4^C587

(Eun et al., 2014)

Detailed Description

Statement

Reference

The adulthood-only expression of E(z)^HMS00066 under the control of Scer\GAL4^C587 and Gal80[ts] leads to adult testes exhibiting significant increases in the numbers of cyst stem cells and of early stage germline cells, as compared to controls.

(Zhang et al., 2017)

Expression of E(z)^HMS00066 under the control of Scer\GAL4^nos.PG results in complete sterility in females. Oogenesis is arrested before the vitellogenic stages, resulting in the presence of degenerating stage 6 egg chambers in the ovary. These mutant egg chambers do not contain an oocyte nucleus, instead having 16 nurse-like cells. At stage 4, the mutant oocytes do not have a compact karyosome and rather resemble nurse cells in size and shape.

Cells undergoing ectopic endocycles (ectopic S phases in the absence of mitotic markers) are seen region 2b of the germarium in females expressing E(z)^HMS00066 under the control of Scer\GAL4^nos.PG.

(Iovino et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions