FB2024_03 , released June 25, 2024
Allele: Dmel\Ube3aC-A.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Ube3aC-A.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0256609
Feature type
allele
Associated gene
Dmel\Ube3a
Associated Insertion(s)
Carried in Construct
P{UAS-Ube3a.C-A}
Also Known As
UAS-Dube3a-C/A
Key Links
Transgenic product class
missense_variant
(Ferdousy et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ferdousy et al., 2011)
Mutagen
in vitro construct
(Ferdousy et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Ube3a.C-A}
Cytology
Description

Amino acid replacement: C?A.

(Ferdousy et al., 2011)

UAS regulatory sequences drive expression of Ube3a coding sequences containing a C to A mutation that renders the construct unable to transfer a ubiquitin molecule to it's substrate.

(Ferdousy et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ube3a
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Ube3aC-A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4repo does not lead to bang-induced, heat-induced or photogenic paralyses; this expression also does not lead to any significant changes in the amplitudes of On transients, Off transients or photoreceptor potential in electroretinograms, as compared to controls.

      (Hope et al., 2017)

      The percentage of larvae with spontaneous depolarizations at the neuromuscular junction is significantly higher than controls in larvae with Ube3aScer\UAS.T:Zzzz\FLAG-51 (but not Ube3aC-A.Scer\UAS.T:Zzzz\FLAG) driven by Scer\GAL4elav-C155.

      (Valdez et al., 2015)

      Overexpression of Ube3aCA.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4GMR.PF results in a rough eye phenotype.

      Expression of Ube3aCA.Scer\UAS.T:Zzzz\FLAG in the nervous system under the control of Scer\GAL4elav-C155 results in an increase in dopamine and a concurrent increase in locomotor activity.

      (Ferdousy et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ube3aC-A.Scer\UAS.T:Zzzz\FLAG
      Ube3aC-A.UAS.Tag:FLAG
      Ube3aCA.Scer\UAS.T:Zzzz\FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (4)