FB2024_03 , released June 25, 2024
Allele: Scer\GAL4Gr33a.4.7
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General Information
Symbol
Scer\GAL4Gr33a.4.7
Species
S. cerevisiae
Name
FlyBase ID
FBal0249481
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Gr33a-GAL4.4.7}
Also Known As
Gr33a-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Weiss et al., 2011)
Progenitor genotype
Carried in construct
P{Gr33a-GAL4.4.7}
Cytology
Description

A 4.7kb Gr33a promoter fragment is fused upstream of sequence encoding a GAL4 driver.

(Weiss et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Gr33a
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      gustatory receptor neuron of terminal organ
      (Kwon et al., 2011)
      gustatory receptor neuron of terminal organ dorsolateral group
      (Kwon et al., 2011)
      gustatory receptor neuron of the epiphysis
      (Kwon et al., 2011)
      larval hypopharyngeal organ gustatory receptor neuron
      (Kwon et al., 2011)
      gustatory receptor neuron of the hypophysis
      (Kwon et al., 2011)
      larval gnathal ganglion
      (Kwon et al., 2011)
      larval gustatory receptor neuron of the epiphysis DP1
      (Apostolopoulou et al., 2016, Choi et al., 2016)
      larval gustatory receptor neuron of the epiphysis DP2
      (Apostolopoulou et al., 2016, Choi et al., 2016)
      larval gustatory receptor neuron of the hypophysis VP1
      (Apostolopoulou et al., 2016, Choi et al., 2016)
      larval gustatory receptor neuron of the hypophysis VP2
      (Apostolopoulou et al., 2016, Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP1
      (Apostolopoulou et al., 2016, Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP2
      (Apostolopoulou et al., 2016, Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP3
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP4
      (Choi et al., 2016)
      abdominal 4 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 5 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 6 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      gustatory receptor neuron B1 of the terminal organ dorsolateral group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron B2 of the terminal organ dorsolateral group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C1 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C2 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C3 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C4 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      adult stage
      high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 5
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 4
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of mesothoracic tarsal segment 5
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of mesothoracic tarsal segment 4
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of metathoracic tarsal segment 5
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of metathoracic tarsal segment 4
      (Ling et al., 2014)
      gustatory receptor neuron | subset of short labellar taste bristle | subset
      (Weiss et al., 2011)
      gustatory receptor neuron | subset of intermediate labellar taste bristle | subset
      (Weiss et al., 2011)
      high salt/bitter-sensing neuron of adult labellum sensillum
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-a
      (Weiss et al., 2011, Weiss et al., 2011)
      intermediate labellar taste bristle
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-b
      (Weiss et al., 2011)
      short labellar taste bristle S-b
      (Weiss et al., 2011)
      short labellar taste bristle S-a | intense
      (Weiss et al., 2011)
      short labellar taste bristle S-b | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle | intense
      (Weiss et al., 2011)
      adult abdominal multidendritic neuron | subset
      (Park and Kwon, 2011)
      short labellar taste bristle S-a
      (Weiss et al., 2011)
      bitter-sensing neuron | subset
      (Israel et al., 2022)
      Additional Information
      Statement
      Reference

      ScerGAL4Gr33a.4.7 drives expression in the following series of neurons. Larval gustatory receptor neuron of the epiphysis DP1, where it is coexpressed with ScerGAL4Gr66a.1.8, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr93a.5, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr9a.6.3, ScerGAL4Gr22d.3.1, ScerGAL4Gr22e.2.5, ScerGAL4Gr28b.a.3, ScerGAL4Gr39a.b.1, ScerGAL4Gr58b.1, and ScerGAL4Gr59d.4.1. Larval gustatory receptor neuron of the epiphysis DP2 where it is coexpressed with ScerGAL4Gr66a.1.8, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr77a.4 and ScerGAL4Gr93b.5.3. Larval gustatory receptor neuron of the hypophysis VP1 and VP2 where it is coexpressed with ScerGAL4Gr66a.1.8 and ScerGAL4Gr68a.PB. Larval gustatory receptor neuron of the hypopharyngeal organ PP1 and PP2 where it is coexpressed with ScerGAL4Gr66a.1.8, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr39a.d.1.7, ScerGAL4Gr93c.3.8 and ScerGAL4Gr93d.2.4 Larval gustatory receptor neuron of the hypopharyngeal organ PP3 where it is coexpressed with ScerGAL4Gr66a.1.8, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr28a.PT, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr39a.d.1.7, ScerGAL4Gr93c.3.8 and ScerGAL4Gr93d.2.4 Larval gustatory receptor neuron of the hypopharyngeal organ PP4 where it is coexpressed with ScerGAL4Gr66a.1.8, ScerGAL4Gr28a.PT, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr39a.d.1.7, ScerGAL4Gr93c.3.8 and ScerGAL4Gr93d.2.4.

      (Choi et al., 2016)

      Expression of UAS- mCD8::GFP via ScerGAL4Gr33a.4.7 confirmed that it is expressed in 12 GRN pairs. These 12 pairs are suggested to represent the entire set of larval bitter receptor neurons. They are: (i) two pairs B1 and B2 in the dorsolateral group of sensilla of the TO, (ii) four pairs C1-C4 in the distal group of TO sensilla, (iii) two pairs D1 and D2 in the DPS (epiphysis), (iv) two pairs E1 and E2 in the VPS (hypophysis), and (v) two pairs F1 and F2 in the PPS (hypopharyngeal organ).

      (Apostolopoulou et al., 2016)

      ScerGAL4Gr33a.4.7 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Expression is also observed in projections to the abdominal ganglion.

      (Park and Kwon, 2011)

      ScerGAL4Gr33a.4.7 is expressed in the gustatory neurons of all of the bitter-sensitive short labellar taste bristles, and the gustatory neurons of all of the intermediate labellar taste bristles.

      (Weiss et al., 2011)

      ScerGAL4Gr33a.4.7 is expressed in all of the short and intermediate bitter-sensitive sensilla of the labellum.

      (Weiss et al., 2011, Weiss et al., 2011)

      ScerGAL4Gr33a.4.7 drives expression in 6 gustatory receptor neurons of the larval terminal organ, two of these having its cell body in the dorsal organ ganglion (TO-dorsolateral group). All 6 neurons co-express ScerGAL4Gr66a.1.8. One of the neurons of the TO-dorsolateral group also expresses ScerGAL4Gr10a.3.7 and ScerGAL4Gr32a.3.7.PW. The other terminal organ neurons express different combinations of drivers. The first neuron expresses ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr28b.e.4.245, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39a.b.1, ScerGAL4Gr59d.4.1, ScerGAL4Gr9a.6.3, ScerGAL4Gr22e.2.5, ScerGAL4Gr22a.4.2, ScerGAL4Gr28b.a.3, ScerGAL4Gr36b.0.9, ScerGAL4Gr36c.2.5, ScerGAL4Gr58b.1, ScerGAL4Gr59a.1.5, ScerGAL4Gr59c.5.5 and ScerGAL4Gr93b.5.3. The second neuron co-expresses ScerGAL4Gr39a.a.3.4, ScerGAL4Gr57a.6, ScerGAL4Gr59d.4.1, ScerGAL4Gr47b.2 and ScerGAL4Gr94a.3.7. The third neuron co-expresses ScerGAL4Gr57a.6 and ScerGAL4Gr97a.1. The fourth neuron expresses ScerGAL4Gr39a.b.1 and ScerGAL4Gr59d.4.1. Expression of ScerGAL4Gr33a.4.7 is also found in two neurons of the dorsal pharyngeal sense organ (DPS or epiphysis), two neurons of the posterior pharyngeal sense organ (PPS or hypopharyngeal sense organ) and in two neurons of the ventral pharyngeal sense organ (VPS or hypophysis).

      (Kwon et al., 2011)
       
      Marker for
      bitter-sensing neuron
      (Israel et al., 2022)
      Reflects expression of
      Gr33a
      (Israel et al., 2022, Apostolopoulou et al., 2016, Ling et al., 2014, Kwon et al., 2011, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
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      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Gr33a.4.7
      Name Synonyms
      Secondary FlyBase IDs
        References (13)