UAS regulatory sequences drive expression of Hsap\TARDBP sequences where the nuclear localization signal (NLS) is mutated.
Expression of Hsap\TARDBPmutNLS.Scer\UAS under the control of Scer\GAL4elav.Switch.PO (in a RU486 background) greatly reduces adult fly longevity (though not as much as expression of the wild-type form).
Expression of Hsap\TARDBPmutNLS.Scer\UAS neuronally under the control of Scer\GAL4elav-C155 at 25[o]C results in pupal lethality.
Glial expression of Hsap\TARDBPmutNLS.Scer\UAS in the developing nervous system under the control of Scer\GAL4repo at 25[o]C results in pupal lethality.
Expression of Hsap\TARDBPmutNLS.Scer\UAS in developing muscle cells under the control of Scer\GAL4how-24B at 25[o]C results in embryonic lethality.
Expression of Hsap\TARDBPmutNLS.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF at 25[o]C results in a severe rough eye phenotype and a loss of eye pigmentation. These phenotypes worsen over time.
Expression of Hsap\TARDBPmutNLS.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF at 29[o]C results in a severe rough eye phenotype, loss of eye pigmentation and necrotic patches. These phenotypes worsen over time.