FlyBase Allele Report: Hsap\TARDBP[M.UAS.Tag:FLAG]

General Information

Symbol

Hsap\TARDBP^{M.UAS.Tag:FLAG}

Species

H. sapiens

Name

FlyBase ID

FBal0248853

Feature type

allele

Associated gene

Hsap\TARDBP

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\TARDBP-FLAG}

Also Known As

UAS-TDP-43

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Miguel et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Hsap\TARDBP-FLAG}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\TARDBP coding sequences.

(Miguel et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\TARDBP

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Miguel et al., 2011)

model of amyotrophic lateral sclerosis type 10

CEC

(Chang and Dubnau, 2023)

CEA

(Azpurua et al., 2021, Couly et al., 2020, Park et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 10

is ameliorated by Hsap\SIGMAR1^UAS.cCa

(Couly et al., 2020)

is ameliorated by RyR^KK101716

(Park et al., 2020)

is ameliorated by Br140^HMC04342

(Azpurua et al., 2021)

is ameliorated by CG2186^HMS01090

(Azpurua et al., 2021)

is ameliorated by CG30379^HMS01567

(Azpurua et al., 2021)

is ameliorated by ash1^HMS00582

(Azpurua et al., 2021)

is ameliorated by br^HMS00042

(Azpurua et al., 2021)

is ameliorated by e(y)3^HMS00337

(Azpurua et al., 2021)

is ameliorated by CG7309^HMC02417

(Azpurua et al., 2021)

is ameliorated by CG8034^HMS00331

(Azpurua et al., 2021)

is ameliorated by enok^HMS02048

(Azpurua et al., 2021)

is ameliorated by enok^HMS02165

(Azpurua et al., 2021)

is ameliorated by mor^HMS01267

(Azpurua et al., 2021)

is ameliorated by CG9451^HMS01016

(Azpurua et al., 2021)

is ameliorated by msk^HMS00020

(Azpurua et al., 2021)

is ameliorated by msk^gRNA.pCFD3-1

(Azpurua et al., 2021)

is ameliorated by msk^gRNA.pCFD3-2

(Azpurua et al., 2021)

is ameliorated by nito^HMS00166

(Azpurua et al., 2021)

is ameliorated by polybromo^HMS00531

(Azpurua et al., 2021)

is ameliorated by tomb^HMS01557

(Azpurua et al., 2021)

is ameliorated by Chd1^HMS01142

(Azpurua et al., 2021)

is exacerbated by Chd1^HMS01142

(Azpurua et al., 2021)

is ameliorated by Gγ30A^HMS01362

(Azpurua et al., 2021)

is ameliorated by Hel25E^HMS00076

(Azpurua et al., 2021)

is ameliorated by Hsp70Bb^HMS00900

(Azpurua et al., 2021)

is ameliorated by MED11^HMS01094

(Azpurua et al., 2021)

is ameliorated by MED15^HMS00522

(Azpurua et al., 2021)

is ameliorated by MED21^HMS01211

(Azpurua et al., 2021)

is ameliorated by MED22^HMS01047

(Azpurua et al., 2021)

is ameliorated by MED27^HMS01050

(Azpurua et al., 2021)

is ameliorated by MED28^HMS00458

(Azpurua et al., 2021)

is ameliorated by MED31^HMS01048

(Azpurua et al., 2021)

is ameliorated by MED8^HMS01275

(Azpurua et al., 2021)

is ameliorated by SF2^HMS00358

(Azpurua et al., 2021)

is ameliorated by SF2^gRNA.pCFD3-1

(Azpurua et al., 2021)

is ameliorated by SF2^gRNA.pCFD3-2

(Azpurua et al., 2021)

is ameliorated by Su(Tpl)^HMS00277

(Azpurua et al., 2021)

is ameliorated by Taf1^HMS00416

(Azpurua et al., 2021)

is ameliorated by Taf9^HMS00336

(Azpurua et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Miguel et al., 2011)

eye | heat sensitive, with Scer\GAL4^GMR.PF

(Miguel et al., 2011)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Miguel et al., 2011)

interommatidial bristle | heat sensitive, with Scer\GAL4^GMR.PF

(Miguel et al., 2011)

ommatidium, with Scer\GAL4^GMR.PF

(Miguel et al., 2011)

ommatidium | heat sensitive, with Scer\GAL4^GMR.PF

(Miguel et al., 2011)

Detailed Description

Statement

Reference

Expression of Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG} neuronally under the control of Scer\GAL4^{elav.Switch.PO} (and RU486 treatment) at 25[o]C results in early death. Examination of the brains of 20 day old mutants fails to identify any obvious abnormalities such as vacuolar pathology.

Expression of Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG} neuronally under the control of Scer\GAL4^elav-C155 at 25[o]C results in L1 larval lethality.

Expression of Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG} in glia in the developing nervous system under the control of Scer\GAL4^repo at 25[o]C results in pupal lethality.

Expression of Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG} in developing muscle cells under the control of Scer\GAL4^how-24B at 25[o]C results in larval lethality.

Expression of Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG} in the developing eye under the control of Scer\GAL4^GMR.PF at 25[o]C results in a mild rough eye phenotype with minor disruption in the external lattice with occasionally interommatidial bristles missing. Expression at 29[o]C results in an additional loss of pigment phenotype. These phenotypes worsen over time.

(Miguel et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG}

Hsap\TARDBP^{M.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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