FlyBase Allele Report: Dmel\Opa1[HMS00349]

FB2024_04 , released June 25, 2024

FB2024_04 , released June 25, 2024

Allele: Dmel\Opa1^HMS00349

General Information

Symbol

Dmel\Opa1^HMS00349

Species

D. melanogaster

Name

FlyBase ID

FBal0248114

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00349}

Also Known As

UAS-Opa1 RNAi

Key Links

Genomic Maps

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00349}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates hypoplastic left heart syndrome

modeled by Chchd3^HMC02376

(Birker et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

abnormal neuroanatomy | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

decreased cell number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased cell number | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

decreased occurrence of cell division | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased occurrence of cell division | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

decreased rate of movement | adult stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

decreased rate of movement | adult stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^elav-C155

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^insc.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^pros.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^sca.PU

(Dubal et al., 2022)

lethal - all die before end of P-stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

Phenotype Manifest In

adult heart, with Scer\GAL4^HCH.Hand

(Birker et al., 2023)

embryonic/larval brain | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

embryonic/larval brain | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

embryonic/larval fat body | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2020)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^Mef2.PU

(Deng et al., 2016)

embryonic/larval neuron | decreased number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

embryonic/larval neuron | decreased number | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

filamentous actin | adult stage, with Scer\GAL4^HCH.Hand

(Birker et al., 2023)

ganglion mother cell | decreased number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

intermediate neuronal progenitor | decreased number | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

intermediate neuronal progenitor | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

mitochondrion | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2020)

mitochondrion | larval stage, with Scer\GAL4^Mhc.PW

(Dubal et al., 2022)

mitochondrion | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

mitochondrion | third instar larval stage, with Scer\GAL4^Mef2.PU

(Deng et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mhc.PW

(Dubal et al., 2022)

muscle cell of A1-7 ventral longitudinal muscle 4 | larval stage, with Scer\GAL4^Mhc.PW

(Dubal et al., 2022)

type II neuroblast | larval stage, with Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

type II neuroblast | larval stage, with Scer\GAL4^wor.PU

(Dubal et al., 2022)

Detailed Description

Statement

Reference

The expression of Opa1^HMS00349 under the control of Scer\GAL4^pnt-14-94 results in type II neuroblasts and daughter mature intermediate precursor cells in the larval brain with fragmented mitochondria. Type II neuroblasts and daughter mature intermediate precursor cells show decreased mitotic index. Mature intermediate precursor cells, ganglion mother cells and young neurons are decreased in number. Type II neuroblast lineages show decreased proliferation (increased S-phase) compared to controls.

The expression of Opa1^HMS00349 under the control of Scer\GAL4^wor.PU results in decreased number of neurons in the larval brain. Type II neuroblasts and neuroblast lineage show decreased mitotic index.

(Dubal et al., 2022)

Expressing Opa1^HMS00349 under the control of Scer\GAL4^Cg.PA leads to small and fragmented mitochondria in the early third instar larval fat body.

(Liu et al., 2020)

Expressing Opa1^HMS00349 under the control of Scer\GAL4^Cg.PA leads to fragmented mitochondria in the third instar larval fat body (as assessed by MitoGFP).

(Zhou et al., 2019)

Expression of Opa1^HMS00349 leads to strong defects in mitochondrial fusion in the body wall cells of third instar larvae (with the Scer\GAL4^Mef2.PU driver) as well as in the indirect flight muscles in adults (with the Scer\GAL4^Mhc.PU driver) - the mitochondria are short and fragmented compared to controls.

(Deng et al., 2016)

Flies expressing Opa1^HMS00349 under the control of Scer\GAL4^GMR.PU do not exhibit any discernible eye phenotype, as compared to controls.

(Hwang et al., 2014)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has abnormal neuroanatomy | larval stage phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has decreased cell number | larval stage phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has decreased occurrence of cell division | larval stage phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^wor.PU has decreased occurrence of cell division | larval stage phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^wor.PU

(Dubal et al., 2022)

Enhancer of

Statement

Reference

Opa1^HMS00349, Scer\GAL4^Cg.PA is an enhancer of abnormal size | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Opa1^HMS00349, Scer\GAL4^hs.2sev is an enhancer of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Suppressor of

Statement

Reference

Opa1^HMS00349/Scer\GAL4^Tub.PU is a suppressor | partially of abnormal cell size | somatic clone | oogenesis phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Opa1^HMS00349/Scer\GAL4^Tub.PU is a suppressor | partially of increased size | somatic clone | oogenesis phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a suppressor | partially of increased cell number | larval stage phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Opa1^HMS00349, Scer\GAL4^Cg.PA has embryonic/larval fat body | early third instar larval stage phenotype, enhanceable by Chchd2^8-8

(Liu et al., 2020)

Opa1^HMS00349, Scer\GAL4^Cg.PA has mitochondrion | early third instar larval stage phenotype, enhanceable by Chchd2^8-8

(Liu et al., 2020)

Suppressed by

Statement

Reference

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has intermediate neuronal progenitor | larval stage | decreased number phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has ganglion mother cell | larval stage | decreased number phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has embryonic/larval neuron | larval stage | decreased number phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has intermediate neuronal progenitor | larval stage phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^wor.PU has type II neuroblast | larval stage phenotype, suppressible by Drp1^SD.UASp, Scer\GAL4^wor.PU

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 has embryonic/larval brain phenotype, suppressible | partially by Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Enhancer of

Statement

Reference

Opa1^HMS00349, Scer\GAL4^Cg.PA is an enhancer of mitochondrion | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Opa1^HMS00349, Scer\GAL4^Cg.PA is an enhancer of embryonic/larval fat body | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Opa1^HMS00349, Scer\GAL4^hs.2sev is an enhancer of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

NOT Enhancer of

Statement

Reference

Opa1^HMS00349/Scer\GAL4^Tub.PU is a non-enhancer of female germ cell nucleus | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Suppressor of

Statement

Reference

Opa1^HMS00349/Scer\GAL4^Tub.PU is a suppressor | partially of follicle cell | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Opa1^HMS00349/Scer\GAL4^Tub.PU is a suppressor | partially of apical part of cell | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a suppressor | partially of embryonic/larval brain phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a suppressor | partially of type II neuroblast | increased number | larval stage phenotype of N^UAS.cMa, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

NOT Suppressor of

Statement

Reference

Opa1^HMS00349/Scer\GAL4^Tub.PU is a non-suppressor of female germ cell nucleus | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a non-suppressor of embryonic/larval brain phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a non-suppressor of type II neuroblast | larval stage phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a non-suppressor of intermediate neuronal progenitor | larval stage phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Opa1^HMS00349, Scer\GAL4^pnt-14-94 is a non-suppressor of mitochondrion | larval stage phenotype of Drp1^SD.UASp, Scer\GAL4^pnt-14-94

(Dubal et al., 2022)

Other

Statement

Reference

Drp1^KG03815, Opa1^HMS00349, Scer\GAL4^Tub.PU has follicle cell | somatic clone phenotype

(Uttekar et al., 2024)

Drp1^KG03815, Opa1^HMS00349, Scer\GAL4^Tub.PU has mitochondrion | somatic clone phenotype

(Uttekar et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\UCP2^Scer\UAS.cFa and Opa1^HMS00349 under the control of Scer\GAL4^GMR.PU does not result in a rough eye phenotype.

(Hwang et al., 2014)

The presence of opa1-like^HMS00349 strongly enhances the reduced adult eye size phenotype found in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies.

(Yang et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

32358

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00349}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Opa1^HMS00349

opa1-like^HMS00349

opa1ⁱ

(Chowdhary et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections