FlyBase Allele Report: Dmel\fwe[UASp.Lose-B]

General Information

Symbol

Dmel\fwe^UASp.Lose-B

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct c of Rhiner

FlyBase ID

FBal0247686

Feature type

allele

Associated gene

Dmel\fwe

Associated Insertion(s)

Carried in Construct

P{UASp-fwe.Lose-B}

Key Links

Transgenic product class

wild_type

(Rhiner et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Rhiner et al., 2010)

Mutagen

in vitro construct

(Rhiner et al., 2010)

Progenitor genotype

Carried in construct

P{UASp-fwe.Lose-B}

Cytology

Description

UASp regulatory sequences drive expression of the "Lose-B" isoform of fwe.

(Rhiner et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

fwe

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

photoreceptor neuron | pupal stage, with Scer\GAL4^elav.PU

(Merino et al., 2013)

photoreceptor neuron | pupal stage | somatic clone, with Scer\GAL4^Act.PU

(Merino et al., 2013)

photoreceptor neuron | pupal stage | somatic clone, with Scer\GAL4^GMR.PU

(Merino et al., 2013)

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

(Rhiner et al., 2010)

Detailed Description

Statement

Reference

Expressing fwe^{Scer\UAS.P\T.Lose-B} in retinal clones under the control of Scer\GAL4^Act.PU induces cell death of pupal photoreceptor neurons.

Retinas ubiquitously expressing fwe^{Scer\UAS.P\T.Lose-B} under the control of Scer\GAL4^Act.PU do not exhibit photoreceptor neuron death. The physiological cell death seen at the periphery of the pupal retina in controls is also not seen. The same is seen when fwe^{Scer\UAS.P\T.Lose-B} is expressed by the eye-specific Scer\GAL4^GMR.PU driver.

Expression of fwe^{Scer\UAS.P\T.Lose-B} in photoreceptor neurons under the control of Scer\GAL4^elav.PU suppresses the physiological cell death seen at the periphery of the pupal retina.

Expressing fwe^{Scer\UAS.P\T.Lose-B} under the control of Scer\GAL4^GMR.PF (but restricting expression in the neuronal cells using Scer\GAL80^elav.PU) does not suppress the physiological cell death seen at the periphery of the pupal retina.

Expressing fwe^{Scer\UAS.P\T.Lose-B} in cone cells under the control of Scer\GAL4^wg.PU does not suppress the physiological cell death seen at the periphery of the pupal retina.

Expressing fwe^{Scer\UAS.P\T.Lose-B} in primary pigment cells and cone cells under the control of Scer\GAL4^wg.PU does not suppress the physiological cell death seen at the periphery of the pupal retina.

(Merino et al., 2013)

Overexpression of fwe^Scer\UAS.cRc driven by Scer\GAL4^Act5C.PI in cell clones triggers apoptosis in the wing imaginal disc epithelium.

(Rhiner et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^Act.PU, fwe^UASp.Lose-B has increased cell death | somatic clone phenotype, non-suppressible by Axn^UAS.cSa, Scer\GAL4^Act.PU

(Merino et al., 2013)

Scer\GAL4^Act5C.PI, fwe^UASp.Lose-B has increased cell death | somatic clone phenotype, non-suppressible by drpr^Δ5

(Rhiner et al., 2010)

Suppressor of

Statement

Reference

fwe^UASp.Lose-B, Scer\GAL4^GMR.PF is a suppressor of increased cell death | larval stage phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

fwe^UASp.Lose-B, Scer\GAL4^GMR.PF is a suppressor of increased cell death | adult stage phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^Act.PU, fwe^UASp.Lose-B has photoreceptor neuron | somatic clone phenotype, non-suppressible by Axn^UAS.cSa, Scer\GAL4^Act.PU

(Merino et al., 2013)

Scer\GAL4^Act5C.PI, fwe^UASp.Lose-B has wing disc | somatic clone phenotype, non-suppressible by drpr^Δ5

(Rhiner et al., 2010)

Suppressor of

Statement

Reference

fwe^UASp.Lose-B, Scer\GAL4^GMR.PF is a suppressor of eye disc phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

fwe^UASp.Lose-B, Scer\GAL4^GMR.PF is a suppressor of adult optic lobe phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PF

(Coelho et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Axn^Scer\UAS.cSb does not suppress the increase in photoreceptor cell death in retinal clones expressing fwe^{Scer\UAS.P\T.Lose-B} under the control of Scer\GAL4^Act.PU.

(Merino et al., 2013)

Cell clones generated in the wing imaginal disc epithelium that are homozygous for drpr^Δ5 and overexpress fwe^Scer\UAS.cRc driven by Scer\GAL4^Act5C.PI still undergo apoptosis.

(Rhiner et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expressing fwe^UASp.Lose-B suppresses the increased adult lobe apoptosis induced by the expression of Hsap\APP^2xAPP.SP.UAS under the control of Scer\GAL4^GMR.PF.

(Coelho et al., 2018)

Complementation and Rescue Data

Fails to rescue

fwe^UASp.Lose-B fails to rescue fwe^e202

(Rhiner et al., 2010)

Comments

Expression of fwe^Scer\UAS.cRc under the control of Scer\GAL4^αTub84B.PL in homozygous fwe^e202 mutant cell clones does not restore the survival of fwe^e202 mutant cells.

(Rhiner et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

fwe^{Scer\UAS.P\T.Lose-B}

fwe^Scer\UAS.cRc

fwe^UASp.Lose-B

Name Synonyms

Saccharomyces cerevisiae UAS construct c of Rhiner

Secondary FlyBase IDs

References (5)

Report Sections

Open Close