UAS regulatory sequences drive expression of an inverted repeat.
The adulthood-only expression of tkvJF01485 under the control of Scer\GAL4esg.PU (in combination with Gal80[ts], for temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; this expression does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4dsx.KI (and Gal80[ts]) leads to a significant decrease in the number of secretory vesicles within accessory gland secondary cells, associated with a significant decrease in the size of the largest multivesicular body/lysosome, as compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen.
The adulthood-only expression of tkvJF01485 under the combined control of Scer\GAL4spi-NP0261 and Gal80[ts] also results in both virgin and mated males showing a significant increase in the number of dense core granule compartments within accessory gland secondary cells, as compared to matched controls.
Knockdown of tkv, through expression of tkvJF01485 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 generates abnormal primordial germ cell spectrosome phenotypes. Approximately 59% of primordial germ cells exhibit wild-type spectrosomes, 21% faint and fragmented spectrosomes, and a further 21% lack spectrosomes altogether.