FB2024_03 , released June 25, 2024
Allele: Scer\GAL4Crz.504.Δ311-249
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General Information
Symbol
Scer\GAL4Crz.504.Δ311-249
Species
S. cerevisiae
Name
FlyBase ID
FBal0244723
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Crz-GAL4.504.Δ311-249}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Choi et al., 2008)
Progenitor genotype
Scer\GAL4Crz.504
(Choi et al., 2008)
Carried in construct
P{Crz-GAL4.504.Δ311-249}
Cytology
Description

Internal deletion of Crz upstream sequences from -311 to -249bp.

(Choi et al., 2008)
Allele components
Component
Use(s)
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      neuron | subset of adult pars lateralis
      (Afonso et al., 2015)
      Additional Information
      Statement
      Reference

      ScerGAL4Crz.504.Δ311-249 drives expression in approximately 14 neurons of the adult pars lateralis that are positive for CycA.

      (Afonso et al., 2015)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Activation of the pars lateralis neurons (neurons expressing Scer\GAL4Crz.504.Δ311-249) decreases sleep. Activity levels during waking periods are not affected. Sleep bout duration is markedly reduced, while sleep bout number shows little change (a significant increase is seen in males but not in females). Sleep latency (the time between lights off to the first sleep) is significantly increased in both males and females.

      Adult-stage activation of Scer\GAL4Crz.504.Δ311-249-expressing neurons also reduces sleep in both make and female flies.

      Blocking the activity of Scer\GAL4Crz.504.Δ311-249-expressing neurons significantly increases sleep in female flies.

      (Afonso et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Crz.504.Δ311-249
      Name Synonyms
      Secondary FlyBase IDs
        References (2)