Almost no third instar larvae expressing Mmp1E225A.f2.Scer\UAS under the control of Scer\GAL4αTub84B.PL display broken tracheal dorsal trunks. 85% survive to pupariation. All dissected pupae show defects in head eversion.
No third instar larvae expressing Mmp1E225A.f2.Scer\UAS under the control of Scer\GAL4btl.PS display broken tracheal dorsal trunks.