FB2024_03 , released June 25, 2024
Allele: Scer\GAL4GABA-B-R2.PR
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General Information
Symbol
Scer\GAL4GABA-B-R2.PR
Species
S. cerevisiae
Name
metabotropic GABA-B receptor subtype 2 promoter construct of Root
FlyBase ID
FBal0243578
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GABA-B-R2-GAL4.R}
Also Known As
GABABR2-Gal4, GABA-B-R2-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Root et al., 2008)
Progenitor genotype
Carried in construct
P{GABA-B-R2-GAL4.R}
Cytology
Description

1.5kb of genomic DNA immediately upstream of the GABA-B-R2 gene is cloned upstream of GAL4 driver coding sequences.

(Root et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
GABA-B-R2_GABABR2-Gal4
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult insulin secreting cell of pars intercerebralis | subset
      (Enell et al., 2010)
      adult pars intercerebralis
      (Enell et al., 2010)
      adult brain | restricted
      (Enell et al., 2010)
      adult antennal lobe | restricted
      (Root et al., 2008)
      ellipsoid body outer central domain | restricted
      (Kahsai et al., 2012)
      ellipsoid body outer posterior domain | restricted
      (Kahsai et al., 2012)
      protocerebral bridge | restricted
      (Kahsai et al., 2012)
      bulb | restricted
      (Kahsai et al., 2012)
      fan-shaped body | restricted
      (Kahsai et al., 2012)
      Additional Information
      Statement
      Reference

      ScerGAL4GABA-B-R2.PR drives expression in the ellipsoid body ring between the innervation regions of R3 and R4d ring neurons and also in the R3 ring. Expression is also detected in the fan-shaped body dorsal and central layers (whereas GABA-B-R2 protein is expressed in all layers), in the protocerebral bridge and bulb (lateral triangle).

      (Kahsai et al., 2012)

      ScerGAL4GABA-B-R2.PR drives expression in the insulin producing cells (IPC), a subset of the median neurosecretory cells of the pars intercerebralis and in other cells located ventrally to the IPC cells that are part of the median neurosecretory cluster. Most, if not all, IPC cells labeled with ScerGAL4GABA-B-R2.PR also express Ilp2 protein.

      (Enell et al., 2010)
       
      Marker for
      Reflects expression of
      GABA-B-R2
      (Kahsai et al., 2012, Enell et al., 2010, Root et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Scer\GAL4GABA-B-R2.PR
      Scer\GAL4GABA-B-R2
      Name Synonyms
      metabotropic GABA-B receptor subtype 2 promoter construct of Root
      Secondary FlyBase IDs
        References (7)