FlyBase Allele Report: Dmel\Shaw[tr.UAS.Tag:FLAG]

General Information

Symbol

Dmel\Shaw^{tr.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0243472

Feature type

allele

Associated gene

Dmel\Shaw

Associated Insertion(s)

Carried in Construct

P{UAS-Shaw.tr.FLAG}

Key Links

Transgenic product class

dominant_negative_variant

(Hodge et al., 2005)

stop_gained

(Hodge et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Hodge et al., 2005)

stop_gained

(Hodge et al., 2005)

Mutagen

in vitro construct

(Hodge et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Shaw.tr.FLAG}

Cytology

Description

UASt regulatory sequences drives expression of a truncated Shaw protein (a stop codon has been introduced at codon 369) which is tagged at the C-terminal end with Tag:FLAG. The protein produced is predicted to be a dominant negative form of Shaw.

(Hodge et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Shaw

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | adult stage, with Scer\GAL4^C164

(Hodge et al., 2005)

abnormal body color | adult stage, with Scer\GAL4^CCAP.PP

(Hodge et al., 2005)

abnormal body color | adult stage, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

abnormal body size | adult stage | female, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

abnormal body size | pharate adult stage, with Scer\GAL4^C164

(Hodge et al., 2005)

abnormal body size | pharate adult stage, with Scer\GAL4^CCAP.PP

(Hodge et al., 2005)

abnormal body size | pharate adult stage, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

abnormal eclosion, with Scer\GAL4^C164

(Hodge et al., 2005)

abnormal eclosion, with Scer\GAL4^CCAP.PP

(Hodge et al., 2005)

abnormal eclosion, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

abnormal neurophysiology | larval stage, with Scer\GAL4^futsch-C380

(Hodge et al., 2005)

chemical sensitive, with Scer\GAL4^C164

(Hodge et al., 2005)

chemical sensitive, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

chemical sensitive, with Scer\GAL4^Mhc.PW

(Hodge et al., 2005)

partially lethal | male, with Scer\GAL4^C164

(Hodge et al., 2005)

partially lethal | male, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

uncoordinated, with Scer\GAL4^C164

(Hodge et al., 2005)

uncoordinated, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

uncoordinated, with Scer\GAL4^Mhc.PW

(Hodge et al., 2005)

visible | adult stage A1, with Scer\GAL4^C164

(Hodge et al., 2005)

visible | adult stage A1, with Scer\GAL4^CCAP.PP

(Hodge et al., 2005)

visible | adult stage A1, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^C164

(Hodge et al., 2005)

adult cuticle, with Scer\GAL4^CCAP.PP

(Hodge et al., 2005)

adult cuticle, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

motor neuron | larval stage, with Scer\GAL4^futsch-C380

(Hodge et al., 2005)

wing, with Scer\GAL4^C164

(Hodge et al., 2005)

wing, with Scer\GAL4^CCAP.PP

(Hodge et al., 2005)

wing, with Scer\GAL4^elav-C155

(Hodge et al., 2005)

Detailed Description

Statement

Reference

Expression of Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} in all clock neurons (under the control of Scer\GAL4^tim.PE) has no clear effect on locomotor rhythms in LD. The same is true when Scer\GAL80^Pdf.PS or Scer\GAL80^cry.PS are added to restrict expression.

Expression of the dominant-negative Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} in the DNs alone, under the control of Scer\GAL4^tim.PE and Scer\GAL80^cry.PS does not result in DD period lengthening.

(Hodge and Stanewsky, 2008)

Males overexpressing Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with Scer\GAL4^C164 or Scer\GAL4^elav-C155 are less viable than female siblings. Some of these adults show an ether-induced shaking phenotype. Some adults show a phenotype with unfurled wings and softened or abnormally tanned cuticle. Some small pupae fail to eclose. These phenotypes are also obtained with high penetrance when driving Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with Scer\GAL4^Ccap.PP.

Flies overexpressing Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with Scer\GAL4^Mhc.PW show an ether-induced shaking phenotype.

Females expressing Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with Scer\GAL4^elav-C155 show a reduced body weight compared to controls.

Expression of Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with Scer\GAL4^C380 depolarises resting membrane potential and increases input resistance of a larval motor neuron (MNISN-Is), decreases the spike rate and increases the delay to first spike. Injection of current to hyperpolarize these neurons to -60mv leads to a significant normalisation of their firing properties, but not to wildtype levels.

(Hodge et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^C164, Shaw^{tr.UAS.Tag:FLAG} has visible | adult stage A1 phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^C164, Shaw^{tr.UAS.Tag:FLAG} has abnormal body size | pharate adult stage phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^C164, Shaw^{tr.UAS.Tag:FLAG} has abnormal eclosion phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^elav-C155, Shaw^{tr.UAS.Tag:FLAG} has partially lethal | male phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^elav-C155, Shaw^{tr.UAS.Tag:FLAG} has visible | adult stage A1 phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^elav-C155, Shaw^{tr.UAS.Tag:FLAG} has abnormal eclosion phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^C164, Shaw^{tr.UAS.Tag:FLAG} has wing phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^C164, Shaw^{tr.UAS.Tag:FLAG} has adult cuticle phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^elav-C155, Shaw^{tr.UAS.Tag:FLAG} has wing phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Scer\GAL4^elav-C155, Shaw^{tr.UAS.Tag:FLAG} has adult cuticle phenotype, enhanceable by Sh¹⁴

(Hodge et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Adults overexpressing Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with either Scer\GAL4^elav-C155 or Scer\GAL4^C164, in combination with Sh¹⁴, show an ether-induced shaking phenotype. Some adults show a phenotype with unfurled wings and softened or abnormally tanned cuticle. Some small pupae fail to eclose.

Males and females expressing Shaw^{tr.Scer\UAS.T:Zzzz\FLAG} with Scer\GAL4^C164, in combination with Sh¹⁴, show reduced body weight compared to controls.

(Hodge et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55748

w^*; P{UAS-Shaw.tr.FLAG}332

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Shaw^{tr.Scer\UAS.T:Zzzz\FLAG}

Shaw^{tr.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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