Flies expressing Rnor\CMR1^Scer\UAS.cPa under the control of Scer\GAL4^c061 and Scer\GAL80^Mef2.PT do not show any differences in appetitive memory performance from controls at 23[o]C. However, shifting these flies to 16[o]C for 1 hour before testing impairs memory.

Flies expressing Rnor\CMR1^Scer\UAS.cPa pan-neuronally under the control of Scer\GAL4^elav-C155 fall down quickly upon transfer to 15[o]C, with animals expressing 3x Rnor\CMR1^Scer\UAS.cPa displaying a more rapid and robust response. Electrophysiological recordings from flight muscles of these animals reveals sustained action potential generation in motoneurons in response to a downward temperature ramp. This effect is readily reversible. Males typically show greater sensitivity than females to a given temperature shift.

Saturating menthol vapor administered to flies expressing Rnor\CMR1^Scer\UAS.cPa pan-neuronally (under the control of Scer\GAL4^elav-C155) at room temperature (24[o]C) causes the animals to seize and fall over as they do in response to cold.

Flies expressing Rnor\CMR1^Scer\UAS.cPa in N[[CCAP]] neurons under the control of Scer\GAL4^Ccap.PP that are shifted briefly to 18[o]C immediately after eclosion rapidly expand their wings relative to controls. They respond the same way to a 15 minute exposure to saturating menthol vapor at room temperature. All flies exhibit an extremely brief grooming phase.

Flies expressing Rnor\CMR1^Scer\UAS.cPa under the control of Scer\GAL4^Ccap.PP in high-perturbation conditions display normal cuticle tanning after N[[CCAP]] activation (indicating normal buriscon release).