FB2024_03 , released June 25, 2024
Allele: Dmel\ArfGAP1UAS.cRa
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General Information
Symbol
Dmel\ArfGAP1UAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Raghu
FlyBase ID
FBal0241092
Feature type
allele
Associated gene
Dmel\ArfGAP1
Associated Insertion(s)
Carried in Construct
P{UAS-ArfGAP1.R}
Key Links
Transgenic product class
wild_type
(Raghu et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Raghu et al., 2009)
Mutagen
in vitro construct
(Raghu et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-ArfGAP1.R}
Cytology
Description

UAS regulatory sequences drive expression of ArfGAP1.

(Raghu et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ArfGAP1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of ArfGAP1Scer\UAS.cRa under the control of Scer\GAL4Hml.Δ results in third instar larval secondary lymph gland lobe overgrowth. Phenotypes are also seen in the primary lobe; an increased number of mitotically active cells is seen in the cortical zone compared to controls and premature differentiation of prohemocytes into plasmatocytes and crystal cells is observed. The number of niche (posterior signalling center) cells is highly reduced compared to controls.

      Expression of ArfGAP1Scer\UAS.cRa under the control of Scer\GAL4Hml.Δ results in endocytic trafficking defects during larval haematopoesis. NICD is trapped in Hrs positive endosomes.

      (Khadilkar et al., 2014)

      Expression of Gap69CScer\UAS.cRa under the control of Scer\GAL4GMR.PF results in a rough eye phenotype. Rhabdomeres develop normally, and there are at best mild endomembrane defects in the cell body.

      Flies expressing Gap69CScer\UAS.cRa under the control of Scer\GAL4GMR.PF and grown in bright light show some defects in rhabdomere structure and some accumulation of endomembranes in the cell body of the photoreceptors.

      (Raghu et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      ArfGAP1UAS.cRa, Scer\GAL4GMR.PF has rhabdomere phenotype, enhanceable by Cds1

      (Raghu et al., 2009)
      Enhancer of
      Statement
      Reference

      ArfGAP1UAS.cRa/Scer\GAL4GMR.PF is an enhancer of rhabdomere phenotype of Cds1

      (Raghu et al., 2009)

      ArfGAP1UAS.cRa/Scer\GAL4GMR.PF is an enhancer of eye photoreceptor cell phenotype of Cds1

      (Raghu et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Gap69CScer\UAS.cRa partially the rhabdomere defects caused by expression of PldScer\UAS.cRa under the control of Scer\GAL4GMR.PF.

      Expression of Gap69CScer\UAS.cRa under the control of Scer\GAL4GMR.PF in a CdsA1 background under bright light illumination results in a dramatic increase in the accumulation of whorls of endomembrane within the cell body, accompanied by poorly formed rhabdomeres.

      (Raghu et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      ArfGAP1Scer\UAS.cRa
      ArfGAP1UAS.cRa
      Gap69CScer\UAS.cRa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Raghu
      Secondary FlyBase IDs
        References (3)