UASt regulatory sequences drive expression of an inverted repeat.
The expression of bratKK113206 under the combined control of Scer\GAL4insc-Mz1407 and Dicer-2 (for efficient RNAi) or under the combined control of Scer\GAL4wor.PA, Scer\GAL80ase.PN and Dicer-2 (for efficient RNAi) frequently leads to brain tumors; in the Scer\GAL4wor.PA, Scer\GAL80ase.PN-regulated expression condition, xenografts of tumor material (either brain pieces or sorted neuroblasts) also induce tumors in the hosts.
FLP-out somatic clones expressing bratKK113206 under the control of Scer\GAL4FRT.Rnor\Cd2.Act5C in the neural compartment of the optic lobe of third instar larvae contain some T4/T5 neurons displaying signs of dedifferentiation. There is no effect on the mode of division of type III neuroblasts.
The expression of bratKK113206 under the combined control of Scer\GAL4wor.PA and Scer\GAL80ase.PN leads to tumors in the larval brain.
The expression of bratKK113206 under the control of Scer\GAL4Toll-6-D42 leads to morphology changes in the third instar larval NMJ, namely satellite boutons instead of the expected "beads on a chain" and a significant increase in the number of active zones, despite of no effect in NMJ volume or synapse number.
Cultured pupal neuroblasts (NBs) from flies expressing bratKK113206 under the control of Scer\GAL4wor.PA and Scer\GAL80ase.PN are able to divide multiple times, but unlike wild type pupal NBs, they remain big and are indistinguishable from larval NBs.
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has short lived phenotype, enhanceable by Scer\GAL4wor.PA/SypGD9477, Scer\GAL80ase.PN
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has neoplasia phenotype, enhanceable by Scer\GAL4wor.PA/SypGD9477, Scer\GAL80ase.PN
bratKK113206 has abnormal neuroanatomy | third instar larval stage | somatic clone phenotype, enhanceable by ato1/ato1
Scer\GAL4insc-Mz1407, bratKK113206 has neoplasia phenotype, suppressible by lncRNA:cherubpromDEL/lncRNA:cherubDEL
Scer\GAL4insc-Mz1407, bratKK113206 has neoplasia phenotype, suppressible by Scer\GAL4insc-Mz1407/lncRNA:cherubRNAi.UAS
Scer\GAL4insc-Mz1407, bratKK113206 has lethal phenotype, suppressible by lncRNA:cherubpromDEL/lncRNA:cherubDEL
Scer\GAL4insc-Mz1407, bratKK113206 has lethal phenotype, suppressible by Scer\GAL4insc-Mz1407/lncRNA:cherubRNAi.UAS
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has short lived phenotype, suppressible by ImpHMS01168, Scer\GAL4wor.PA, Scer\GAL80ase.PN
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has short lived phenotype, suppressible by Scer\GAL4wor.PA/SypRB.UAS.Tag:HA, Scer\GAL80ase.PN
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has neoplasia phenotype, suppressible by ImpHMS01168, Scer\GAL4wor.PA, Scer\GAL80ase.PN
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has neoplasia phenotype, suppressible by Scer\GAL4wor.PA/SypRB.UAS.Tag:HA, Scer\GAL80ase.PN
Scer\GAL4insc-Mz1407, bratKK113206 has neoplasia phenotype, non-suppressible by lncRNA:cherub+tLa, lncRNA:cherubDEL/lncRNA:cherubDEL
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has brain phenotype, enhanceable by Scer\GAL4wor.PA/SypGD9477, Scer\GAL80ase.PN
bratKK113206 has T4 neuron | third instar larval stage | somatic clone phenotype, enhanceable by ato1/ato1
bratKK113206 has T5 neuron | third instar larval stage | somatic clone phenotype, enhanceable by ato1/ato1
Scer\GAL4insc-Mz1407, bratKK113206 has brain phenotype, suppressible by lncRNA:cherubpromDEL/lncRNA:cherubDEL
Scer\GAL4insc-Mz1407, bratKK113206 has brain phenotype, suppressible by Scer\GAL4insc-Mz1407/lncRNA:cherubRNAi.UAS
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has brain phenotype, suppressible by ImpHMS01168, Scer\GAL4wor.PA, Scer\GAL80ase.PN
Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratKK113206 has brain phenotype, suppressible by Scer\GAL4wor.PA/SypRB.UAS.Tag:HA, Scer\GAL80ase.PN
Scer\GAL4Toll-6-D42, bratKK113206 has neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4Toll-6-D42/orb2KK101114
Scer\GAL4insc-Mz1407, bratKK113206 has brain phenotype, non-suppressible by lncRNA:cherub+tLa, lncRNA:cherubDEL/lncRNA:cherubDEL
Scer\GAL4Toll-6-D42/bratKK113206 is a suppressor of synapse | third instar larval stage phenotype of Scer\GAL4Toll-6-D42, orb2KK101114
Scer\GAL4Toll-6-D42/bratKK113206 is a suppressor of neuromuscular junction | third instar larval stage phenotype of Scer\GAL4Toll-6-D42, orb2KK101114
bratKK113206 is not rescued by Scer\GAL4wor.PA/bratUAS.Tag:FLAG.Tag:MYC/Scer\GAL80ase.PN
The larval brain tumor phenotype observed upon the expression of bratKK113206 under the combined control of Scer\GAL4wor.PA and Scer\GAL80ase.PN is rescued by the co-expression of either bratScer\UAS.RNAi-res.T:Zzzz\FLAG.T:Hsap\MYC or bratG774D.Scer\UAS.RNAi-res.T:Zzzz\FLAG.T:Hsap\MYC, but not of bratScer\UAS.T:Zzzz\FLAG.T:Hsap\MYC.