UASt regulatory sequences drive expression of an inverted repeat.
At 18[o]C, nearly all animals expressing Klp64DKK100214 under the control of Scer\GAL4nub-AC-62 show mild defects in the distal part of the wings. Most of the defects seen comprise notching in the wing margin and loss of margin bristles. Phenotypes are temperature-dependent: at 29[o]C, flies can almost develop to the adult but die in the pupal case. These flies show severe shrinkage or complete loss of wing.
Expressing Klp64DKK100214 at and near the DV boundary region using Scer\GAL4bbg-C96 causes notching along the wing margin. This phenotype is seen with 100% penetrance.