FB2024_03 , released June 25, 2024
Allele: Dmel\lsnNN31
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General Information
Symbol
Dmel\lsnNN31
Species
D. melanogaster
Name
FlyBase ID
FBal0230472
Feature type
allele
Associated gene
Dmel\lsn
Associated Insertion(s)
Carried in Construct
Also Known As
vps22NN31
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Vaccari et al., 2009)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C?T.

(Vaccari et al., 2009)

Amino acid replacement: Q137term.

(Vaccari et al., 2009)

The premature stop codon is predicted to result in a truncated protein that lacks the C-terminal 117 amino acids of the wild-type product.

(Vaccari et al., 2009)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:21,865,686..21,865,686 [+]
Nucleotide change:

C21865686T

Reported nucleotide change:

C?T

Amino acid change:

Q137term | lsn-PA

Reported amino acid change:

Q137term

(Vaccari et al., 2009)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous lsnNN31 mutant tissue partially survives in mosaic eye discs, and is not completely 'out-competed'. Although mutant ommatidia are not recovered, the wild-type ommatidia composing the adult eyes of mosaic animals are variably overgrown.

      Compared with wild-type, egg chambers containing lsnNN31 mutant cells exhibit a loss of plasma membrane, ultimately leading to the formation of clusters of multinucleated cells enclosed by a single actin cytoskeleton, with nuclei rested around clumps of actin-positive structures, possibly also membrane fragments.

      lsnNN31 mutant oocytes rarely contain intralumenal vesicles and isolated multivesicular bodies. They also exhibit irregularly shaped yolk granules.

      (Vaccari et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      lsnNN31
      vps22NN31
      (Wang et al., 2021, Vaccari et al., 2009)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)