FlyBase Allele Report: Dmel\BEAF-32[NP6377]

General Information

Symbol

Dmel\BEAF-32^NP6377

Species

D. melanogaster

Name

FlyBase ID

FBal0223596

Feature type

allele

Associated gene

Dmel\BEAF-32

Associated Insertion(s)

P{GawB}BEAF-32^NP6377

Carried in Construct

Also Known As

BEAF^NP6377

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Gurudatta et al., 2012)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}BEAF-32^NP6377

Cytology

Description

Insertion in a protein coding exon common to both BEAF-32 A and B isoforms.

(Gurudatta et al., 2012)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,771,824..14,771,824 2R:14,771,826..14,771,826

(Hart, 2014, Gurudatta et al., 2012, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage (with Df(2R)BSC429)

(Jukam et al., 2016)

abnormal neuroanatomy | adult stage | somatic clone

(Jukam et al., 2016)

Phenotype Manifest In

photoreceptor cell R8 of pale ommatidium (with Df(2R)BSC429)

(Jukam et al., 2016)

photoreceptor cell R8 of pale ommatidium | somatic clone

(Jukam et al., 2016)

photoreceptor cell R8 of yellow ommatidium (with Df(2R)BSC429)

(Jukam et al., 2016)

photoreceptor cell R8 of yellow ommatidium | somatic clone

(Jukam et al., 2016)

Detailed Description

Statement

Reference

BEAF-32^NP6377/Df(2R)BSC429 animals and homozygous BEAF-32^NP6377 retinal clones (covering the entire retina) have an increased proportion of pale R8 photoreceptors and a reciprocally decreased proportion of yellow R8 photoreceptors, compared to controls.

(Jukam et al., 2016)

FlyBase curator comment: FBrf0219796 reports that the chromosome carrying the P{GawB}BEAF-32^NP6377 insertion is homozygous lethal and causes neoplastic growth phenotypes. A subsequent report (FBrf0224638) provides evidence that these phenotypes are not due to an effect on BEAF-32, but are instead due to second-site mutations on the chromosome carrying the P{GawB}BEAF-32^NP6377 insertion.

(Gurudatta et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

105221

y^* w^*; P{GawB}BEAF-32^NP6377 / CyO, P{UAS-lacZ.UW14}UW14

Notes on Origin

Discoverer

The homozygous larval lethality of the NP6377 chromosome is not due to the P{GawB}BEAF-32^NP6377 insertion in BEAF-32, but is instead due to second site mutation(s): the lethality of the NP6377 chromosome is not rescued by BEAF-32^+t5 or BEAF-32^EGFP, and BEAF-32^AB-KO (a null allele of BEAF-32) complements the lethality of the NP6377 chromosome.

(Hart, 2014)

Flies carrying BEAF-32^AB-KO over the NP6377 chromosome were found to be viable and could be maintained as a healthy stock over 25 generations. This was unexpected as BEAF-32^AB-KO/BEAF-32^AB-KO homozygous females are almost sterile (see FBrf0200670). This suggests that in addition to the second site lethality, the NP6377 chromosome also carries another second site mutation: a dominant mutation that suppresses the female fertility defect caused by a lack of BEAF-32 protein. FlyBase curator comment: this second site dominant suppressor of BEAF-32 mutant female sterility has subsequently been named Tofu¹ (see FBrf0255826).

(Hart, 2014)

The two second site mutations on the NP6377 chromosome (lethality and dominant suppressor of BEAF-32 mutant female sterility) are separable and therefore do not appear to be caused by the same mutation.

(Hart, 2014)

Separable from: Tofu¹.

(McKowen and Hart, 2022)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

BEAF-32^NP6377

(Jukam et al., 2016, Gurudatta et al., 2012)

BEAF^NP6377

(McKowen and Hart, 2022, Hart, 2014)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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