FB2024_03 , released June 25, 2024
Allele: Avic\GFPEGFP.ppk.1
Open Close
General Information
Symbol
Avic\GFPEGFP.ppk.1
Species
A. victoria
Name
FlyBase ID
FBal0221331
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{ppk-EGFP.1}
Also Known As
ppk-EGFP, ppk-GFP, ppk-EGFP5, pickpocket-EGFP
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kuo et al., 2005, Grueber et al., 2003)
Progenitor genotype
Carried in construct
P{ppk-EGFP.1}
Cytology
Description

A ~1kb fragment from the ppk enhancer region fused to a minimal Hsp70 promoter drives expression of EGFP.

(Grueber et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
ppk
ppk_1kb
Encoded product / tool
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 16 -- late third instar larval stage
      larval multidendritic class IV neuron
      (Parrish et al., 2009)
      embryonic stage 16 -- P-stage
      dendritic arborizing neuron | subset
      (Grueber et al., 2003)
      third instar larval stage
      larval multidendritic class IV neuron | subset
      (Song et al., 2011)
      larval dorsal multidendritic neuron ddaC
      (Song et al., 2011)
      abdominal anterior ventral multidendritic neuron vdaa
      (Song et al., 2011)
      larval ventral multidendritic neuron vdaB
      (Song et al., 2011)
      adult stage
      neuron | subset of lateral oviduct

      Comment: 2 neurons

      (Yang et al., 2009)
      neuron | subset of uterus

      Comment: 30 neurons

      (Yang et al., 2009)
      neuron | subset of ejaculatory duct

      Comment: 7 neurons

      (Yang et al., 2009)
      neuron | subset of antenna
      (Yang et al., 2009)
      neuron | subset of wing margin
      (Yang et al., 2009)
      neuron | subset of leg
      (Yang et al., 2009)
      neuron | subset of adult abdomen

      Comment: body wall

      (Yang et al., 2009)
      adult stage | female
      adult ventral nerve cord | restricted
      (Rezaval et al., 2012)
      neuron | subset of lateral oviduct

      Comment: 2 neurons

      (Rezaval et al., 2012)
      neuron | subset of uterus

      Comment: 15 neurons

      (Rezaval et al., 2012)
      fruitless expressing neuron | subset of uterus
      (Häsemeyer et al., 2009)
      sensory neuron | subset of uterus
      (Häsemeyer et al., 2009)
      sensory neuron | subset of oviduct
      (Häsemeyer et al., 2009)
      adult sex peptide sensory neuron of uterus
      (Häsemeyer et al., 2009)
      adult sex peptide sensory neuron of lateral oviduct
      (Häsemeyer et al., 2009)
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval multidendritic class IV neuron
      (Morikawa et al., 2011)
      Additional Information
      Statement
      Reference

      AvicGFP1.E.ppk drives expression in 4 clusters of neurons in the adult female genital tract: one cluster of 2 neurons in the lateral oviduct (with one neuron co-localising with ScerGAL4dsx.KI and the other with EcollexAfru.P1.T:HsimVP16) and three clusters in the uterus. An anterior one with 3 neurons (which also express ScerGAL4dsx.KI) and one more posterior with 7 neurons which co-express ScerGAL4dsx.KI, with 3 neurons of those also expressing EcollexAfru.P1.T:HsimVP16. The most posterior cluster in the uterus is composed of 5 neurons.

      (Rezaval et al., 2012)

      Signal is observed in the axonal terminal branches of of class IV dendritic arborizing (C4da) neurons in the ventral nerve cord as a ladder-like pattern with a longitudinal fascicle on each side and a commissural fascicle in every segment.

      (Morikawa et al., 2011)

      AvicGFPEGFP.ppk.1 is expresssed in 2-3 ScerGAL4fru.P1.D positive fruitless expressing neurons either side of the uterus.

      (Häsemeyer et al., 2009)

      In females, AvicGFP1.E.ppk drives expression in 2 neurons on each lateral oviduct and 15 neurons on each side of the uterus, organised in 3 clusters. Eight of these reproductive tract neurons labeled by AvicGFP1.E.ppk co-express ScerGAL4fru.P1.D. In males, AvicGFP1.E.ppk labels 7 neurons in the ejaculatory duct, with two of these co-expressing ScerGAL4fru.P1.D.

      (Yang et al., 2009)

      This reporter reproduces the expression of the ppk gene in Type IV dendritic aborising neurons. There is also a low level of expression in some Type III neurons.

      (Grueber et al., 2003)
       
      Marker for
      dendritic arborizing neuron
      (Grueber et al., 2003)
      larval multidendritic class IV neuron
      (Morikawa et al., 2011, Emoto et al., 2006, Kuo et al., 2006)
      Reflects expression of
      ppk
      (Rezaval et al., 2012, Song et al., 2011, Häsemeyer et al., 2009, Parrish et al., 2009, Yang et al., 2009, Grueber et al., 2003)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Avic\GFP1.E.ppk
      Avic\GFP5.E.ppk
      Avic\GFPEGFP.ppk.1
      Name Synonyms
      pick-pocket-EGFP
      (Morikawa et al., 2011)
      Secondary FlyBase IDs
      • FBal0148470
      • FBal0190823
      References (27)