Expression of Dh44^JF01822 under the control of Scer\GAL4^Dh44 leads to lower secretion rate of Malpighian tubules in post-injury condition.

Expression of Dh44^JF01822 under the control of Scer\GAL4^GMR65C11 significantly increases adult survival time under desiccation, but not starvation, conditions; there is a slight decrease in adult body weight, but water content is unchanged relative to controls; there is no effect on baseline Malpighian tubule secretion rate or the ability of tubules to respond to Dh44 stimulation.

Expression of Dh44^JF01822 under the control of Scer\GAL4^nSyb.PU in females results in a large, significant decrease in the latency to sperm and mating plug ejection, from >1 hour in controls to within 10 mins after the end of copulation (AEC), a significant reduction in the number of eggs laid in the 48h period AEC, and a significant decrease in the mating refractory period AEC.

Expression of Dh44^JF01822 under the control of Scer\GAL4^nSyb.PU in females does not result in any change in copulation duration, and eggs produced from these females are fertile and develop to adulthood normally.

Expression of Dh44^JF01822 under the control of Scer\GAL4^elav-C155 (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) results in a degradation of circadian locomotor activity rhythms under constant darkness conditions compared to controls.