FB2024_03 , released June 25, 2024
Allele: Dmel\Rab10T23N.UASp.YFP
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General Information
Symbol
Dmel\Rab10T23N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215408
Feature type
allele
Associated gene
Dmel\Rab10
Associated Insertion(s)
Carried in Construct
P{UASp-YFP.Rab10.T23N}
Also Known As
Rab10DN
Key Links
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
in vitro construct
(Zhang et al., 2007, Zhang et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-YFP.Rab10.T23N}
Cytology
Description

Amino acid replacement: T23N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab10 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rab10
Tagged with
YFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Animals expressing Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4grh.D4 are viable, no obvious defects in larval dorsal trunk elongation and airway morphology are observed and the larvae are of comparable size to age-matched controls.

      (Wingen et al., 2017)

      Male control flies fed methamphetamine (3 or 6 mg/ml) have significantly increased activity, whereas Scer\GAL4Appl.G1a>Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP flies only show a slight but significant increase in activity at 6 mg/ml (not 3 mg/ml); Scer\GAL4ChAT.7.4>Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP flies show a highly significant increase in activity when fed 3mg/ml methamphetamine.

      Flies with expression of Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP driven by Scer\GAL4Appl.G1a (but not Scer\GAL4ChAT.7.4) survive longer than wild type flies fed 3 or 6 mg/ml methamphetamine.

      Unlike control flies, Scer\GAL4Appl.G1a>Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP flies do not show a significant increase in activity in response to caffeine; there is no effect on caffeine-induced mortality.

      (Vanderwerf et al., 2015)

      The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4Hml.Δ are not significantly different from those seen in control hemocytes.

      (Garg and Wu, 2014)

      Expression of Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4tj-NP1624 results in the production of round eggs.

      (Lerner et al., 2013)

      Expression of Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4ninaE.PT does not result in light-dependent retinal degeneration (flies raised under 12 hour light/12 our dark conditions show a normal electroretinogram amplitude at 3 weeks of age).

      (Xiong et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rab10T23N.Scer\UAS.P\T.T:Avic\GFP-YFP
      Rab10T23N.UASp.YFP
      Rab10T23N
      (Hertenstein et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)