FlyBase Allele Report: Dmel\Rab8[T22N.UASp.YFP]

General Information

Symbol

Dmel\Rab8^{T22N.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215402

Feature type

allele

Associated gene

Dmel\Rab8

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab8.T22N}

Also Known As

Rab8DN

Key Links

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab8.T22N}

Cytology

Description

Amino acid replacement: T22N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab8 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab8

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Delfino et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

follicle cell, with Scer\GAL4^GR1

(Devergne et al., 2017)

ovarian basement membrane | oogenesis, with Scer\GAL4^GR1

(Devergne et al., 2017)

Detailed Description

Statement

Reference

The expression of Rab8^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GR1 leads to the deposition of basement membrane components on both basal and apical extracellular sides of the follicle cell, as compared to only on the basal side in controls.

(Devergne et al., 2017)

The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus by adult hemocytes expressing Rab8^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Hml.Δ are not significantly different from those seen in control hemocytes.

(Garg and Wu, 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Rab8^{T22N.UASp.YFP}, Scer\GAL4^elav-C155 is a non-enhancer of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Suppressor of

Statement

Reference

Rab8^{T22N.UASp.YFP}/Scer\GAL4^GMR57C10 is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

NOT Suppressor of

Statement

Reference

Rab8^{T22N.UASp.YFP}, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{T22N.UASp.YFP}, Scer\GAL4^elav-C155 is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{T22N.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab8^{T22N.UASp.YFP}, Scer\GAL4^elav-C155 is an enhancer of rhabdomere | decreased number phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^{T22N.UASp.YFP}, Scer\GAL4^elav-C155 is an enhancer of eye phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab8^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 partially rescues the bang sensitivity of para^bss1/+ flies, but does not rescue the bang sensitivity of hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9780

y¹ w^*; P{UASp-YFP.Rab8.T22N}12

23271

y¹ w^*; P{UASp-YFP.Rab8.T22N}vimar⁰⁹/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab8^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab8^{T22N.UASp.YFP}

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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