FB2024_03 , released June 25, 2024
Allele: Dmel\sideI1563
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General Information
Symbol
Dmel\sideI1563
Species
D. melanogaster
Name
FlyBase ID
FBal0212946
Feature type
allele
Associated gene
Dmel\side
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Meyer and Aberle, 2006)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q222term.

(Heymann et al., 2022)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:27,398,967..27,398,967 [-]
Nucleotide change:

C27398967T

Amino acid change:

Q222term | side-PA; Q222term | side-PB; Q222term | side-PC; Q222term | side-PD

Reported amino acid change:

Q222term

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Heymann et al., 2022)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      growth cone & intersegmental nerve (with sideC137)

      (Siebert et al., 2009)

      larval intersegmental nerve (with sideC137)

      (Siebert et al., 2009)

      larval intersegmental nerve (with sideC137), with Scer\GAL4btl.PS, sideUAS.cSa

      (Siebert et al., 2009)

      larval intersegmental nerve (with sideC137), with Scer\GAL4srp, sideUAS.cSa

      (Siebert et al., 2009)

      larval neuromuscular junction & abdominal dorsal acute muscle 1 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal dorsal acute muscle 2 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal dorsal oblique muscle 1 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal dorsal oblique muscle 2 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal lateral transverse muscle 1 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal lateral transverse muscle 2 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal lateral transverse muscle 3 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal lateral transverse muscle 4 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral acute muscle 1 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral acute muscle 2 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral acute muscle 3 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral longitudinal muscle 1 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral longitudinal muscle 2 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral longitudinal muscle 3 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral longitudinal muscle 4 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral oblique muscle 4 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral oblique muscle 5 (with sideC137)

      (Meyer and Aberle, 2006)

      larval neuromuscular junction & abdominal ventral oblique muscle 6 (with sideC137)

      (Meyer and Aberle, 2006)

      muscle cell of A1-7 dorsal acute muscle 1 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 dorsal acute muscle 2 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 dorsal oblique muscle 1 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 dorsal oblique muscle 2 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 ventral longitudinal muscle 1 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 ventral longitudinal muscle 2 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 ventral longitudinal muscle 3 (with sideC137)

      (Siebert et al., 2009)

      muscle cell of A1-7 ventral longitudinal muscle 4 (with sideC137)

      (Siebert et al., 2009)
      Detailed Description
      Statement
      Reference

      49% of hemisegments show detachment of ISN motor axons from sensory nerves of the anterior fascicle in sideC137/sideI1563 embryos.

      sideC137/sideI1563 embryos show severe delays in the dorsal migration of the ISN compared to wild type. Growth cones do eventually manage to cross the dorsal trunk, but a subset lag behind. The average growth rate of the mutant nerve is 15.2 +/- 4.6 mm/h compared to 31.9 +/- 13.4 mm/h for wild-type nerves. A small fraction of mutant ISN growth cones show highly disoriented directionality, extending in abnormal directions over the course of axonal growth.

      Most, if not all, ISN growth cones are tightly attached to tracheal branches in sideC137/sideI1563 embryos expressing sideScer\UAS.cSa in the trachea under the control of Scer\GAL4btl.PS. The ISN follows the tracheal substrate in these embryos and crosses and re-crosses the transverse connective.

      Most, if not all, ISN growth cones are attached to hemocytes in sideC137/sideI1563 embryos expressing sideScer\UAS.cSa in the hemocytes under the control of Scer\GAL4srp (this does not occur in wild-type embryos). Filopodial extensions of both cell membranes are tightly interdigitated.

      sideC137/sideI1563 third instar larvae often lack neuromuscular junctions (NMJs) on dorsal muscles (the percentage of muscles lacking NMJs is given in parentheses); muscle 1 (19%), muscle 9 (24%), muscle 2 (11%), muscle 10 (9%).

      sideC137/sideI1563 third instar larvae often lack neuromuscular junctions (NMJs) on ventral muscles (the percentage of muscles lacking NMJs is given in parentheses); muscle 12 (58%), muscle 13 (75%), muscle 6 (77%), muscle 7 (85%).

      (Siebert et al., 2009)

      The ISN motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 25.0% (muscle 1), 35.0% (muscle 9), 16.7% (muscle 2), 11.7% (muscle 10).

      The SNa motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 41.7% (muscle 21), 3.4% (muscle 22), 3.3% (muscle 23), 51.7% (muscle 24).

      The ISNb motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 75.0% (muscle 12), 75.0% (muscle 13), 83.3% (muscle 6), 85.0% (muscle 7).

      The SNc motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 61.7% (muscle 26), 86.7% (muscle 27), 66.7% (muscle 29).

      The ISNd motor nerve of sideC137/sideI1563 third instar larvae shows innervation defects at the neuromuscular junction on a number of muscles (percentage of hemisegments with defects on the indicated muscle is listed); 78.3% (muscle 15), 63.3% (muscle 16), 98.4% (muscle 17).

      (Meyer and Aberle, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: A mutation affecting the structure of the neuromuscular junction.

      (Meyer and Aberle, 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sideI1563
      (Heymann et al., 2022, Kinold et al., 2018, Siebert et al., 2009, Meyer and Aberle, 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)