Embryos expressing SAK^{Scer\UAS.cRMa} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} do not develop and become progressively full of free centrosomes which nucleate free asters of microtubules not associated with spindles. The centrosomes first appear in 15- to 3-minute-old embryos and spread to fill the entire embryo after 2 to 3 hours. The spreading of the centrosomes follows the wild-type spindle axial expansion pattern. The centrioles in these embryos are structurally normal and procentrioles are observed next to completed ones.

The sperm aster around the incoming basal body and the first mitotic spindle are normal in early embryos expressing SAK^{Scer\UAS.cRMa} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, but at anaphase or telophase of the first mitosis, more than two centrosomes are seen at each pole (indicating the onset of centrosome amplification).

Meiosis II occurs normally in unfertilised eggs expressing SAK^{Scer\UAS.cRMa} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}. However, eggs that have exited meiosis II contain free centrosomes. The centrosomes appear scattered at random positions within the egg, including at the anterior and posterior poles. The formation of the first centrioles appears to start later in eggs than in embryos, suggesting that centrioles take longer to be made in the absence of a template. The centrioles in these eggs are structurally normal and procentrioles are observed next to completed ones.