FlyBase Allele Report: Dmel\Diap1[RNAi.UAS.cHa]

General Information

Symbol

Dmel\Diap1^RNAi.UAS.cHa

Species

D. melanogaster

Name

FlyBase ID

FBal0212214

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

P{UAS-Diap1.RNAi.H}

Key Links

Transgenic product class

RNAi_reagent

(Huh et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Huh et al., 2007)

Mutagen

in vitro construct

(Huh et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Diap1.RNAi.H}

Cytology

Description

UAS regulatory sequences drive expression of a mir-6 microRNA in which 21bp within the mir-6 precursor backbone have been replaced by sequences complementary to Diap1.

(Huh et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Diap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

increased cell death | third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

Phenotype Manifest In

autophagosome | somatic clone | third instar larval stage, with Scer\GAL4^Act.PU

(Santhanam et al., 2014)

gastric caecum | third instar larval stage, with Scer\GAL4^Act.PU

(Santhanam et al., 2014)

head | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

leg | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

peptidergic neuron | third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

pupal cuticle | posterior, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

wing disc | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

Detailed Description

Statement

Reference

The clonal expression of Diap1^{miRNA.Scer\UAS} under the control of Scer\GAL4^Act.PU results in an increase in the number of autophagosomes, identified as fluorescent Atg8a puncta, in the gastric caecum cells of third instar larvae, as compared to controls.

(Santhanam et al., 2014)

Expression of th^{miRNA.Scer\UAS} under the control of Scer\GAL4^CCAP.PP compromises metamorphic development of the offspring, producing retracted posterior cuticle, failure of head formation, and incomplete extension of imaginal wings and legs. Premature CCAP neuron cell death is seen in the third instar larval CNS.

(Lee et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

C. Chen

(Huh et al., 2007)

B. Hay

(Huh et al., 2007)

Comments

This construct is expected to produce a microRNA targeting the th gene.

(Huh et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Diap1^RNAi.UAS.cHa

Diap1^{miRNA.Scer\UAS}

Diap1^miRNA.UAS

th^{miRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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